Large-scale analysis of chlorophyll fluorescence kinetics in Synechocystis sp PCC 6803:: Identification of the factors involved in the modulation of photosystem stoichiometry
Large-scale analysis of chlorophyll fluorescence kinetics in Synechocystis sp PCC 6803:: Identification of the factors involved in the modulation of photosystem stoichiometry
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DOI：
10.1093/pcp/pcm015
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发表时间：
2007-03-01
影响因子：
4.9
通讯作者：
Sonoike, Kintake
中科院分区：
文献类型：
--
作者：
Ozaki, Hiroshi;Ikeuchi, Masahiko;Sonoike, Kintake
由于叶绿素荧光反映了光合电子传递链的氧化还原状态，对叶绿素荧光的监测已成功应用于光合作用相关基因的筛选。本文报道了通过叶绿素荧光诱导动力学的简单比较，可以识别出在光系统化学计量调节方面存在缺陷的突变体。我们建立了一个包含500个转座子介导基因破坏的蓝细菌聚囊藻（Synechocystis sp. PCC 6803）突变体的文库，并对这些突变体进行了45 s的叶绿素荧光动力学测量。我们找到了两个参与光系统化学计量调节的基因pmgA和sll1961。这两个基因的干扰物在荧光动力学上有共同的特征，我们寻找表现出这种特征的突变体。在目前鉴定的6个突变体中，5个在强光条件下表现出不同的光系统化学计量。因此，基于荧光动力学相似性的分类是识别基因功能的一种极好的方法。
Since chlorophyll fluorescence reflects the redox state of photosynthetic electron transport chain, monitoring of chlorophyll fluorescence has been successfully applied for the screening of photosynthesis-related genes. Here we report that the mutants having a defect in the regulation of photosystem stoichiometry could be identified through the simple comparison of the induction kinetics of chlorophyll fluorescence. We made a library containing 500 mutants in the cyanobacterium Synechocystis sp. PCC 6803 with transposon-mediated gene disruption, and the mutants were used for the measurement of chlorophyll fluorescence kinetics for 45 s. We picked up two genes, pmgA and sll1961, which are involved in the modulation of photosystem stoichiometry. The disruptants of the two genes share common characteristics in their fluorescence kinetics, and we searched for mutants that showed such characteristics. Out of six mutants identified so far, five showed a different photosystem stoichiometry under high-light conditions. Thus, categorization based on the similarity of fluorescence kinetics is an excellent way to identify the function of genes.