Gene expression profiling of rice grown in free air CO2 enrichment (FACE) and elevated soil temperature
Gene expression profiling of rice grown in free air CO2 enrichment (FACE) and elevated soil temperature
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DOI：
10.1016/j.fcr.2010.11.018
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发表时间：
2011-02-28
影响因子：
5.8
通讯作者：
Miyao, Mitsue
中科院分区：
文献类型：
--
作者：
Fukayama, Hiroshi;Sugino, Miho;Miyao, Mitsue
大气中二氧化碳浓度的上升伴随着全球变暖。然而，二氧化碳升高和土壤温度升高的相互作用对植物的影响尚未得到充分研究。在这里，我们通过微阵列研究了自由空气 CO2 富集（FACE，环境 +20 Pa）、升高的土壤温度（+2 摄氏度）或两者的组合对水稻第 10 个旗叶发育和完全展开的旗叶基因表达的影响。基因表达受到 FACE 的显着影响，并且在每个实验条件下鉴定出 400-600 个上调或下调的基因。总体而言，土壤温度升高可能会增加不同表达基因的数量。其中，无论叶期和土壤温度如何，只有 31 个上调基因和 83 个下调基因是常见的。这些包括蔗糖转运蛋白和果糖基转移酶的上调以及叶绿素a/b结合蛋白、硝酸盐转运蛋白和水通道蛋白的下调，尽管大多数不同表达的基因在功能上是未知的。尽管存在一些例外，但 FACE 并未显着改变大多数初级代谢基因的表达。在FACE下，与CO2固定相关的基因（例如Rubisco和碳酸酐酶）的表达下调，而与蔗糖合成、糖酵解、TCA循环和N固定相关的大部分基因的表达上调，尽管在所有情况下变化都很小。这些结果可能反映了代谢对 FACE 的转录适应，降低了 CO2 固定能力并刺激了暗呼吸。 (C) 2010 Elsevier B.V. 保留所有权利。
Rising atmospheric CO2 concentration is accompanied by global warming. However, interactive effects of elevated CO2 and soil temperature have not been well studied in plants. Here, we studied the effects of free air CO2 enrichment (FACE, ambient +20 Pa), elevated soil temperature (+2 degrees C), or a combination of both on gene expression in developing 10th and fully expanded flag leaves of rice by microarray. Gene expression was significantly affected by FACE and 400-600 up-regulated or down-regulated genes were identified in each experimental condition. Overall, elevated soil temperature was likely to increase the number of differently expressed genes. Among them, only 31 up-regulated and 83 down-regulated genes were common irrespective of the leaf stage and soil temperature. These included up-regulation of sucrose transporter and fructosyltransferase and down-regulation of chlorophyll a/b binding protein, nitrate transporter and aquaporin, though most of differently expressed genes were functionally unknown. Although there were some exceptions, the expression of most of primary metabolism genes was not significantly altered by FACE. The expression of genes related to CO2 fixation such as Rubisco and carbonic anhydrase were down-regulated, whereas a large proportion of genes related to sucrose synthesis, glycolysis, TCA cycle and N fixation were up-regulated under FACE, though changes were small in all cases. These results might reflect the transcriptional adaptation of metabolism to FACE, reducing the CO2 fixation capacity and stimulating the dark respiration. (C) 2010 Elsevier B.V. All rights reserved.