Cryptococcus nanyangensis sp. nov., A New Basidiomycetous Yeast Isolated from the Gut of Wood-Boring Larvae
Cryptococcus nanyangensis sp. nov., A New Basidiomycetous Yeast Isolated from the Gut of Wood-Boring Larvae
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DOI：
10.1007/s00284-012-0203-7
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发表时间：
2012-08
影响因子：
2.6
通讯作者：
F. Hui;Qiuhong Niu;T. Ke;Yingxi Li
中科院分区：
文献类型：
--
作者：
F. Hui;Qiuhong Niu;T. Ke;Yingxi Li
从中国中部宝天曼自然保护区的钻木幼虫肠道中分离到两株担子菌新种。对大亚基(LSU)rRNA基因的D_1/D_2区和内转录间隔区(ITS)的序列分析表明，这些酵母菌与11个未被描述的隐球菌种形成了一个簇。该新种与其最接近的已知种隐球菌属D1D2区的差异为3.3%(15个取代位，630个碱基以上的缺口)，ITS区的差异为13.4%(508个碱基以上的41个取代位和27个缺口)。生理学上观察到该新酵母菌株在杜伦管中发酵葡萄糖、半乳糖、蔗糖、海藻糖和棉子糖。根据表型和分子特征，这两个菌株被认为是一个新种--南洋隐球菌属。模式菌株KCY-1T(=CICC 1976T=CBS 12474T)。
Two strains of a novel basidiomycetous yeast species were isolated from the gut of wood-boring larvae collected in the Baotianman Nature Reserve, the central China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) regions showed that these yeasts belong to theBulleromycesclade and formed a cluster together with eleven undescribedCryptococcusspecies. The novel species differed from its closest known species,Cryptococcusrajasthanensis, by 3.3 % divergence (15 substitutions and 6 gaps over 630 bases) in the D1/D2 domains, and by 13.4 % divergence (41 substitutions and 27 gaps over 508 bases) in the ITS regions. Physiologically, the fermentation of glucose, galactose, sucrose, trehalose, and raffinose in Durham tubes was observed for the strains of this new yeast. Based on the phenotypical and molecular characteristics presented, the two strains are proposed as a new species,Cryptococcus nanyangensissp. nov., with the type strain KCY-1T(=CICC 1976T= CBS 12474T).