Stat3 participates in the metabotropic glutamate signaling pathway in Bergmann glial cells
Stat3 participates in the metabotropic glutamate signaling pathway in Bergmann glial cells
复制标题
DOI：
10.1023/a:1021044424103
复制
发表时间：
1999-08-01
影响因子：
4.4
通讯作者：
Ortega, A
中科院分区：
文献类型：
--
作者：
Aguilera, P;Ortega, A
浦肯野细胞与小脑中的平行纤维建立谷氨酸能突触。伯格曼神经胶质细胞围绕着这个突触。转录因子 Stat3 在小脑中高度表达，存在于小鸡伯格曼神经胶质细胞培养物中。用 L-谷氨酸处理这些细胞会诱导 Stat3 的酪氨酸磷酸化。 15 分钟后观察到最大磷酸化，30 分钟后恢复至基础水平。这种效应与 Stat3 易位到细胞核以及凝胶迁移率变化测定中延迟条带的出现相关。 L-谷氨酸观察到的作用是通过代谢型谷氨酸受体介导的。这些结果进一步支持了这样的观点，即神经胶质细胞通过改变现有蛋白质以及基因表达调控来对L-谷氨酸作出反应。
Purkinje cells establish a glutamatergic synapse with the parallel fibers in the cerebellum. Bergmann glial cells surround this synapse. The transcription factor Stat3, highly expressed in cerebellum, is present in cultures of chick Bergmann glia. Treatment of these cells with L-glutamate induces the tyrosine-phosphorylation of Stat3. The maximal phosphorylation was observed after 15 min and by 30 min returned to basal levels. This effect correlated with Stat3 translocation to nucleus and the appearance of a retarded band in gel mobility shift assays. The effects observed with L-glutamate were mediated through metabotropic glutamate receptors. These results further support the notion that glial cells respond to L-glutamate through changes in the existing proteins and also in gene expression regulation.