Identification and expression of bovine Ucp1 variants
Identification and expression of bovine Ucp1 variants
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DOI：
10.1016/j.bbalip.2022.159111
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发表时间：
2022-02-01
影响因子：
4.8
通讯作者：
Funaba, Masayuki
中科院分区：
文献类型：
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作者：
Diao, Zhicheng;Murakami, Masaru;Funaba, Masayuki
棕色/米色脂肪细胞暴露在寒冷中会以热量的形式释放化学能[1]。解偶联蛋白(UCP)1主要在棕色/米色脂肪细胞中表达，主要负责能量消耗[1]。由于功能性棕色/米色脂肪细胞存在于成人中[1]，这些细胞可能成为预防和治疗人类肥胖的靶细胞。我们以前观察到成熟牛的白色脂肪中存在棕色/米色脂肪细胞[2]。我们还发现UCP1在牛骨骼肌组织和肌源性细胞中意外表达[3]。虽然少量脂肪细胞普遍存在于牛骨骼肌中，但这些脂肪细胞不表达UCP1，肌源性细胞表达UCP1[3]。在这项研究中，我们研究了牛UCP1基因的表达。动物护理和实验是由Azabu大学动物护理委员会批准的(140902-4、160516-9、160516-10和190306-3)，并按照批准的指南进行。在Azabu大学兽医教学医院住院的12头牛获得了13个脂肪库(表S1)。我们之前的研究中使用了一个样本(样本1)[3]。
Brown/beige adipocytes dissipate chemical energy as heat when exposed to cold [1]. Uncoupling protein (UCP) 1, predominantly expressed in brown/beige adipocytes, is primarily responsible for energy expenditure [1]. Because functional brown/beige adipocytes occur in adult humans [1], these cells may be a target for preventing and treating human obesity. We previously observed the presence of brown/beige adipocytes in the white fat of mature cattle [2]. We also demonstrated unexpected expression of UCP1 in cattle skeletal muscle tissues and myogenic cells [3]. Although small numbers of adipocytes are generally resident in bovine skeletal muscles, these adipocytes were negative for UCP1 and myogenic cells expressed UCP1 [3]. In this study, we characterized the expression of the bovine Ucp1 gene.Animal care and experiments were approved by the Animal Care Committee of Azabu University (140902-4, 160516-9, 160516-10, and 190306-3), and were conducted in accordance with the approved guidelines. Thirteen fat depots from 12 cattle hospitalized in the Azabu University Veterinary Teaching Hospital were obtained (Table S1). One sample (sample# 1) was used in our previous study [3].