Analysis of the proportion and neuronal activity of excitatory and inhibitory neurons in the rat dorsal spinal cord after peripheral nerve injury
Analysis of the proportion and neuronal activity of excitatory and inhibitory neurons in the rat dorsal spinal cord after peripheral nerve injury
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DOI：
10.1016/j.neulet.2021.135707
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发表时间：
2021-02-21
影响因子：
2.5
通讯作者：
Sakai, Akinori
中科院分区：
文献类型：
--
作者：
Motojima, Yasuhito;Ueta, Yoichi;Sakai, Akinori
背侧脊髓包含将体感信息传递到大脑的投射神经元和然后调节这些投射神经元和/或其他中间神经元的神经元活动的中间神经元。中间神经元可分为两类：兴奋性神经元和抑制性神经元。虽然抑制性中间神经元被认为在镇痛中起关键作用，但尚不清楚它们是否参与神经病理性疼痛。在本研究中，我们的目的是评估的比例和兴奋性/抑制性神经元在脊髓背侧神经病理性疼痛大鼠模型的神经元活性。部分坐骨神经结扎（PSNL）后，大鼠表现出显着的机械性痛觉过敏，随后进行免疫组织化学研究，在板层I-III的背侧脊髓。我们发现，FosB免疫反应阳性细胞的数量显着增加;有没有变化的百分比在NeuN阳性神经元的Pax 2阳性/阴性神经元; Pax 2阴性神经元，但不是Pax 2阳性神经元，主要激活PSNL大鼠;和钙通道α 2 δ 1亚基的免疫荧光强度显着较高。这些结果表明，虽然周围神经损伤可能不会影响兴奋性和抑制性神经元的比例，它主要激活兴奋性神经元在板层I-III的大鼠脊髓背侧。
The dorsal spinal cord contains projection neurons that transmit somatosensory information to the brain and interneurons which then modulate neuronal activity of these projection neurons and/or other interneurons. Interneurons can be subdivided into two groups: excitatory and inhibitory neurons. While inhibitory interneurons are thought to play a crucial role in analgesia, it is unclear whether they are involved in neuropathic pain. In the present study, we aimed to assess the proportion and neuronal activity of excitatory/inhibitory neurons in the dorsal spinal cord using a neuropathic pain model in rats. Following partial sciatic nerve ligation (PSNL), rats showed significant mechanical hyperalgesia, and subsequent immunohistochemical studies were conducted in laminae I-III of the dorsal spinal cord. We found that the number of FosB-immunoreactive cells was significantly higher; there was no change in the percentage of Pax2 positive/negative neurons in NeuN positive neurons; Pax2 negative neurons, but not Pax2 positive neurons, were predominantly activated in PSNL rats; and the immunofluorescence intensity of the calcium channel alpha 2 delta 1 subunit was significantly higher. These results indicate that while peripheral nerve injury might not affect the proportion of excitatory and inhibitory neurons, it predominantly activates excitatory neurons in laminae I-III of the rat dorsal spinal cord.