Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia
Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia
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DOI：
10.1371/journal.ppat.1000047
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发表时间：
2008-04-01
期刊：
影响因子：
6.7
通讯作者：
Nichol, Stuart T.
中科院分区：
文献类型：
--
作者：
Delgado, Simon;Erickson, Bobbie R.;Nichol, Stuart T.
2003年12月和2004年1月，在玻利维亚科恰班巴附近发生了小范围的出血热病例。仅从1例死亡病例中获得标本，其临床病程包括发热、头痛、关节痛、肌痛和呕吐，随后病情恶化和多种出血体征。从两例患者血清样本中分离出一种非细胞病变病毒，并用兔多价抗血清进行IFA染色，鉴定为一种沙粒病毒，该抗血清针对已知与HF （Guanarito， Machupo和Sabia）相关的南美沙粒病毒。RT-PCR分析和随后的完整病毒S和L RNA片段序列分析确定该病毒属于新世界B支沙粒病毒，其中包括所有致病性南美沙粒病毒。该病毒与Sabia病毒的亲缘关系最为密切，但S和L段的核苷酸差异分别为26%和30%，L、Z、N和GP蛋白的氨基酸差异分别为26%、28%、15%和22%，表明该病毒是一种新发现的沙粒病毒，我们建议将其命名为Chapare病毒。总之，两种不同的沙粒病毒Machupo和Chapare可能与玻利维亚的严重HF病例有关。
A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs. A non-cytopathic virus was isolated from two of the patient serum samples, and identified as an arenavirus by IFA staining with a rabbit polyvalent antiserum raised against South American arenaviruses known to be associated with HF (Guanarito, Machupo, and Sabia). RT-PCR analysis and subsequent analysis of the complete virus S and L RNA segment sequences identified the virus as a member of the New World Clade B arenaviruses, which includes all the pathogenic South American arenaviruses. The virus was shown to be most closely related to Sabia virus, but with 26% and 30% nucleotide difference in the S and L segments, and 26%, 28%, 15% and 22% amino acid differences for the L, Z, N, and GP proteins, respectively, indicating the virus represents a newly discovered arenavirus, for which we propose the name Chapare virus. In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia.