Expression of mRNA for natriuretic peptide receptor subtypes in bovine kidney.
Expression of mRNA for natriuretic peptide receptor subtypes in bovine kidney.
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牛肾中利钠肽受体亚型 mRNA 的表达。
DOI：
10.1152/ajprenal.1994.267.2.f318
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Wilson,CB
中科院分区：
文献类型：
--
作者：
Yamamoto,T;Feng,L;Mizuno,T;Hirose,S;Kawasaki,K;Yaoita,E;Kihara,I;Wilson,CB
观察心钠素(ANP)受体亚型(A、B、C)在肾脏中的分布。核糖核酸酶保护实验定量分析显示，肾小球A型受体(ANPRA)和内髓A型受体(ANPRA)分别为6.9×10(7)和10.4×10(7)个/微克总RNA，C型受体(ANPRC)为21.7×10(7)个/微克总RNA。B型受体(ANPRB)mRNA在肾脏各部分均匀分布，数量较少(4.5-4.9×10(6)分子/微克总RNA)。原位杂交显示ANPRA和ANPRC在肾小球上皮细胞中选择性表达，ANPRA在内髓集合管细胞中表达。ANPRC也被定位在肾小球上皮细胞的足突上，通过免疫组织化学方法使用该受体的特异性抗体。这些结果表明，ANPRA是肾脏ANP家族激素的主要生物活性受体，并选择性地存在于肾小球上皮细胞和内髓集合管细胞上。这些细胞被认为在ANP家族诱导的肾小球滤过率和钠排泄的调节中发挥作用。
The localization of mRNA for atrial natriuretic peptide (ANP) receptor subtypes (A, B, C) in the kidney was examined. Quantitative analysis of the ribonuclease protection assay showed that the numbers of type A receptor (ANPRA) mRNA were 6.9 x 10(7) in the glomeruli and 10.4 x 10(7) molecules/micrograms of total RNA in the inner medulla, and that of type C receptor (ANPRC) mRNA was 21.7 x 10(7) molecules/micrograms of total RNA in the glomeruli. The type B receptor (ANPRB) mRNA was present in smaller numbers (4.5-4.9 x 10(6) molecules/micrograms of total RNA) evenly throughout the kidney fractions. In situ hybridization demonstrated both ANPRA and ANPRC mRNA selectively in the glomerular epithelial cells and ANPRA mRNA in the collecting duct cells of the inner medulla. ANPRC was also localized on the foot processes of glomerular epithelial cells by immunohistochemistry using a specific antibody against the receptor. These results indicate that ANPRA is the major biologically active receptor for the ANP family of hormones in the kidney and is present selectively on the glomerular epithelial cells and inner medullary collecting duct cells. These cells are presumed to play a role in the regulation of glomerular filtration rate and sodium excretion induced by the family of ANP.