Characterizing the extracellular and intracellular fluorescent products of activated sludge in a sequencing batch reactor
Characterizing the extracellular and intracellular fluorescent products of activated sludge in a sequencing batch reactor
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表征序批式反应器中活性污泥的细胞外和细胞内荧光产物
DOI：
10.1016/j.watres.2008.03.010
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发表时间：
2008-06-01
期刊：
影响因子：
12.8
通讯作者：
Yu, Han-Qing
中科院分区：
文献类型：
--
作者：
Li, Wei-Hua;Sheng, Guo-Ping;Yu, Han-Qing
采用三维激发-发射矩阵（EEM）荧光光谱法对序批式反应器（SBR）中活性污泥的胞外和胞内物质进行了表征。采用平行因子分析（PARAFAC）从重叠光谱中提取纯光谱。从细胞外物质中鉴定出三种主要成分：蛋白质、黄腐质和腐殖质样物质。它们的荧光峰分别位于280/350、340/400和390/450 nm的激发/发射（Ex/Em）处。细胞外蛋白的荧光与废水化学需氧量的变化规律相似，黄腐酸样物质在一个循环中变化不明显，腐殖酸样物质在底物摄取阶段积累，随后减少。蛋白质和烟酰胺腺嘌呤二核苷酸，还原形式（NADH），被确定为两个主要的细胞内荧光团，其荧光峰（Ex/Em）分别在280/340和350/450 nm。细胞内荧光团的荧光强度评分与生物反应器的性能密切相关。因此，本研究结果为EEM荧光光谱技术在废水处理活性污泥系统监测中的潜在应用奠定了基础。(c) 2008 Elsevier Ltd.版权所有。
Three-dimensional excitation-emission-matrix (EEM) fluorescence spectrometry was used to characterize the extracellular and intracellular substances of activated sludge in a sequencing batch reactor (SBR). Parallel factor analysis (PARAFAC) was applied to extract the pure spectra from the overlapped spectra. Three main components, proteins, fulvic and humic-like substances, were identified from the extracellular substances. Their fluorescence peaks were at an excitation/emission (Ex/Em) of 280/350, 340/400 and 390/450 nm, respectively The fluorescence of the extracellular proteins had a similar changing pattern with the wastewater chemical oxygen demand, the fulvic-like substance did not vary significantly in a cycle and the humic-like substances accumulated in the substrate uptake phase but decreased later. Proteins and nicotinamide adenine dinucleotide, reduced form (NADH), were identified as the two main intracellular fluorophores, and their fluorescence peaks (Ex/Em) were at 280/340 and 350/450 nm, respectively. The fluorescence intensity scores of the intracellular fluorophores were closely related to the bioreactor performance. Thus, the results of this work provide a foundation for potential utilization of the EEM fluorescence spectroscopy to monitor the activated sludge systems for wastewater treatment. (c) 2008 Elsevier Ltd. All rights reserved.