EXTRACELLULAR-MATRIX DERIVED FROM HAIR AND SKIN FIBROBLASTS STIMULATES HUMAN SKIN MELANOCYTE TYROSINASE ACTIVITY
EXTRACELLULAR-MATRIX DERIVED FROM HAIR AND SKIN FIBROBLASTS STIMULATES HUMAN SKIN MELANOCYTE TYROSINASE ACTIVITY
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DOI：
10.1111/j.1365-2133.1994.tb08586.x
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发表时间：
1994-12-01
影响因子：
10.3
通讯作者：
MACNEIL, S
中科院分区：
文献类型：
--
作者：
BUFFEY, JA;MESSENGER, AG;MACNEIL, S
有间接证据表明，皮肤和头发黑色素细胞都受邻近细胞活动的调节。在头发中，特化的成纤维细胞（真皮乳头细胞）似乎在头发生长的调节中起作用。头发色素沉着可能与头发生长有关。在皮肤中，黑色素细胞位于基底膜区附近。据我们所知，成纤维细胞与黑素细胞的直接相互作用尚未被研究过。因此，本研究的目的是建立共培养条件，以研究来自皮肤或头发的真皮成纤维细胞是否会影响黑素细胞的分化。研究了成纤维细胞条件培养基、与成纤维细胞共培养以及成纤维细胞来源的细胞外基质（ECM）对正常人皮肤黑素细胞酪氨酸酶活性的影响。皮肤和头发的成纤维细胞能够改变黑素细胞的形态，并显著增加酪氨酸酶的活性，当黑素细胞在没有主要增殖驱动的情况下培养时。虽然在条件培养基和成纤维细胞共培养中可以检测到酪氨酸酶活性的刺激，但最显著的结果是在成纤维细胞产生的ECM中获得的，平均而言，在6天内酪氨酸酶活性增加了4倍。因此，该研究描述了共培养条件，其中可以检查成纤维细胞对黑素细胞分化的刺激作用。
There is indirect evidence that both skin and hair melanocytes are regulated by the activity of adjacent cells. In hair, the specialized fibroblasts (dermal papilla cells) appear to play a role in the regulation of hair growth. Hair pigmentation may relate to hair growth. In skin, melanocytes are located adjacent to the basement membrane zone. As far as we are aware, direct interactions of fibroblasts with melanocytes have not previously been investigated. Accordingly, the objective of this study was to develop co-culture conditions in which to investigate whether dermal fibroblasts from skin or hair could influence melanocyte differentiation. The influence of fibroblast-conditioned media, co-culture with fibroblasts, and fibroblast-derived extracellular matrix (ECM) on normal human skin melanocyte tyrosinase activity was examined. Fibroblasts from both skin and hair were capable of altering melanocyte morphology and significantly increasing tyrosinase activity when melanocytes were cultured in the absence, but not the presence, of the major proliferative drives. Although stimulation of tyrosinase activity was detectable with conditioned medium and co-culture with fibroblasts, the most striking result was obtained with the fibroblast-produced ECM which, on average, produced a four-fold increase in tyrosinase activity within 6 days. Thus, the study describes co-culture conditions in which the stimulatory effect of the fibroblast on melanocyte differentiation can be examined.