Peptide nanofibers modified with a protein by using designed anchor molecules bearing hydrophobic and functional moieties.
Peptide nanofibers modified with a protein by using designed anchor molecules bearing hydrophobic and functional moieties.
复制标题
通过使用带有疏水性和功能性部分的设计锚定分子对蛋白质进行修饰的肽纳米纤维。
作者：
Ayaka Miyachi;Tsuyoshi Takahashi;S. Matsumura;H. Mihara
多肽和蛋白质的自组装是生物功能的一个关键特征。具有β-折叠结构的短两亲性多肽可以形成复杂的纳米纤维结构，这些纤维可以作为纳米材料来排列生物分子。如我们以前的工作所报道的，多肽FI(H-PKFKIIEFEP-OH)自组装成具有卷曲精细结构的纳米纤维。我们构建了锚定分子，既有纤维结构的结合部分，又有能够捕获目标分子的功能单元，目的是在设计的多肽纳米纤维上排列蛋白质。设计的以烷基链为结合单元、生物素为功能基团的锚链可与多肽纤维FI和F2I(H-ALEAKFAAFEAKLA-NH(2))结合。暴露在纤维表面的生物素部分可以捕获抗生物素抗体。此外，由连接到Phe-Phe或Ile-Ile上的亚氨基二乙酸酯和连接到生物素上的6个组氨酸残基组成的疏水二肽锚定单元也可以通过Ni(2+)离子的螯合将FI多肽纤维连接到抗生物素抗体上。这种使用设计锚定的策略开启了在多肽纳米材料上构建纳米级蛋白质阵列的新方法。
Self-assembly of peptides and proteins is a key feature of biological functions. Short amphiphilic peptides designed with a beta-sheet structure can form sophisticated nanofiber structures, and the fibers are available as nanomaterials for arranging biomolecules. Peptide FI (H-PKFKIIEFEP-OH) self-assembles into nanofibers with a coiled fine structure, as reported in our previous work. We have constructed anchor molecules that have both a binding moiety for the fiber structure and a functional unit capable of capturing target molecules, with the purpose of arranging proteins on the designed peptide nanofibers. Designed anchors containing an alkyl chain as a binding unit and biotin as a functional moiety were found to bind to peptide fibers FI and F2i (H-ALEAKFAAFEAKLA-NH(2)). The surface-exposed biotin moiety on the fibers could capture an anti-biotin antibody. Moreover, hydrophobic dipeptide anchor units composed of iminodiacetate connected to Phe-Phe or Ile-Ile and a peptide composed of six histidine residues connected to biotin could also connect FI peptide fibers to the anti-biotin antibody through the chelation of Ni(2+) ions. This strategy of using designed anchors opens a novel approach to constructing nanoscale protein arrays on peptide nanomaterials.
影响因子：
2.9
作者：
Shtilerman, MD;Ding, TT;Lansbury, PT
通讯作者：
Lansbury, PT