Distinct Hippocampal Expression Profiles of Long Non-coding RNAs in an Alzheimer's Disease Model.
Distinct Hippocampal Expression Profiles of Long Non-coding RNAs in an Alzheimer's Disease Model.
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阿尔茨海默病模型中长非编码 RNA 的独特海马表达谱
DOI：
10.1007/s12035-016-0038-5
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发表时间：
2017-09
影响因子：
5.1
通讯作者：
Peng W
中科院分区：
文献类型：
--
作者：
Yang B;Xia ZA;Zhong B;Xiong X;Sheng C;Wang Y;Gong W;Cao Y;Wang Z;Peng W
阿尔茨海默病(AD)是世界范围内最常见的痴呆形式，是一种以记忆和其他认知功能进行性丧失为特征的复杂的神经退行性疾病。阿尔茨海默病的发病机制尚不完全清楚。尽管最近发现长非编码RNA(Long Non-Coding RNAs，LncRNAs)在AD发病机制中起作用，但LncRNAs在AD中的具体作用仍不清楚，尤其是缺乏AD大鼠海马区LncRNA的表达谱。本研究采用基因芯片技术研究AD模型大鼠海马区异常表达的lncRNAs的表达模式。在AD模型中，共有315个lncRNAs和311mRNAs被发现表达显著失调(≥2.0倍，p<0.05)。然后，用实时定量聚合酶链式反应验证所选的lncRNAs和mRNAs的表达。生物信息学工具和数据库被用来探索潜在的lncRNA功能。本研究首次全面发现AD大鼠海马区异常表达的lncRNAs，并阐明不同的lncRNA表达模式在AD发病机制中的作用。这些信息将有助于进一步研究AD的发病机制，并促进针对lncRNAs的新型AD治疗药物的开发。
Alzheimer’s disease (AD), the most prevalent form of dementia worldwide, is a complex neurodegenerative disease characterized by the progressive loss of memory and other cognitive functions. The pathogenesis of AD is not yet completely understood. Although long non-coding RNAs (lncRNAs) have recently been shown to play a role in AD pathogenesis, the specific influences of lncRNAs in AD remain largely unknown; in particular, hippocampal lncRNA expression profiles in AD rats are lacking. In this study, microarray analysis was performed to investigate the hippocampal expression patterns of dysregulated lncRNAs in a rat model of AD. A total of 315 lncRNAs and 311 mRNAs were found to be significantly dysregulated in the AD model (≥2.0 fold, p < 0.05). Then, quantitative real-time PCR was used to validate the expression of selected lncRNAs and mRNAs. Bioinformatics tools and databases were employed to explore the potential lncRNA functions. This is the first study to comprehensively identify dysregulated hippocampal lncRNAs in AD and to demonstrate the involvement of different lncRNA expression patterns in the hippocampal pathogenesis of AD. This information will enable further research on the pathogenesis of AD and facilitate the development of novel AD therapeutics targeting lncRNAs.
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4
作者：
Lee, Doo Young;Moon, Jangsup;Chu, Kon
通讯作者：
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影响因子：
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