Interleukin-1β activity and collagen synthesis in human dental pulp fibroblasts
Interleukin-1β activity and collagen synthesis in human dental pulp fibroblasts
复制标题
DOI：
10.1097/00004770-200203000-00003
复制
发表时间：
2002-03-01
影响因子：
4.2
通讯作者：
Hussain, MZ
中科院分区：
文献类型：
--
作者：
Barkhordar, RA;Ghani, QP;Hussain, MZ
免疫病理反应在牙髓炎性疾病中起重要作用。白细胞介素-1 β（IL-1 β）被认为是介导细胞免疫应答的关键参与者。在这项研究中，我们测量了IL-1 β的含量及其对健康和患病牙髓成纤维细胞胶原合成的影响。我们发现，与健康的牙髓成纤维细胞相比，患病的牙髓成纤维细胞含有2.5倍的IL-1 β，合成的胶原蛋白量高出80%。然而，外源性IL-1 β未能刺激患病成纤维细胞的胶原合成，而健康牙髓成纤维细胞的胶原合成被刺激超过2倍。这些观察结果表明，牙髓疾病诱导与成纤维细胞对IL-1 β的反应相关的异常。
Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from healthy and diseased dental pulps. We found that diseased pulp fibroblasts contain 2.5-fold greater amounts of IL-1beta and synthesized 80% greater amounts of collagen compared with healthy pulp fibroblasts. However, exogenous IL-1beta failed to stimulate collagen synthesis by diseased fibroblasts, whereas collagen synthesis by healthy pulp fibroblasts was stimulated by more than 2-fold. These observations imply that pulp disease induces abnormalities associated with fibroblast response toward IL-1beta.