Physical parameters of hydroxyapatite adsorption and effect on candidacidal activity of histatins
Physical parameters of hydroxyapatite adsorption and effect on candidacidal activity of histatins
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DOI：
10.1016/s0003-9969(03)00012-8
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发表时间：
2003-05-01
影响因子：
3
通讯作者：
Oppenheim, FG
中科院分区：
文献类型：
--
作者：
Yin, A;Margolis, HC;Oppenheim, FG
组胺素1、3和5是存在于人类唾液分泌物中的富含组氨酸的蛋白质家族的主要成员。这些蛋白质与许多唾液蛋白质的不同之处在于它们在中性pH下的高正电荷密度以及它们的抗细菌和抗真菌特性。在这项研究中，羟基磷灰石吸附特性，histatin 1，含有一个单一的磷酸丝氨酸残基，重组表达histatin 1，天然histatin 3，合成histatin 5和histatin 5的内部12个残基序列进行了研究。朗缪尔模型用于分析吸附。磷酸化和重组组蛋白1的亲和力和结合位点的比较提供了单个磷酸丝氨酰的积极影响的估计。矿物质吸附组。此外，一个明显的相关性被证明存在于肽链长度和结合位点的数量之间。通过用组胺5和组胺5/羟基磷灰石悬浮液进行白色念珠菌杀灭试验，研究了组胺5吸附对其抗念珠菌活性的影响。随着羟基磷灰石含量的增加，杀伤活性降低。结果表明，抗念珠菌性质的组胺素5可能会受到损害的构象所造成的矿物质吸附，或假定的细胞受体所必需的杀念珠菌。当组胺素5被吸附在羟基磷灰石上时，活性是不可接近的。(C)2003爱思唯尔科技有限公司版权所有。
Histatins 1, 3 and 5 are the major members of a histidine-rich protein family present in human salivary secretions. These proteins are distinct from many salivary proteins in their high positive charge density at neutral pH, and their antibacterial and antifungal properties. In this study, the hydroxyapatite adsorption characteristics of, histatin 1, containing a single phosphoserine residue, recombinantly expressed histatin 1, native histatin 3, synthetic histatin 5 and an internal 12-residue sequence of histatin 5 were investigated. A Langmuir-type model. was used to analyse the adsorption. A comparison of the affinities and binding sites of phosphorylated and recombinant histatin 1 provided an estimate of the positive influence of the single phosphoseryl. group on mineral adsorption. Furthermore, an apparent correlation was shown to exist between peptide chain length and the number of binding sites. The influence of histatin 5 adsorption on its anticandidal activity was also investigated by performing Candida albicans killing assays with histatin 5 and histatin 5/hydroxyapatite suspensions. A decrease in killing activity was observed with the increase of hydroxyapatite present. The results suggest that the anticandidal properties of histatin 5 could be impaired by the conformations resulting from mineral adsorption, or that putative cellular receptors necessary for candidacidal. activity are inaccessible when histatin 5 is adsorbed on hydroxyapatite. (C) 2003 Elsevier Science Ltd. All rights reserved.