Intestinal transfer of short‐chain fatty acids in vitro
Intestinal transfer of short‐chain fatty acids in vitro
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短链脂肪酸的体外肠道转移
DOI：
10.1113/jphysiol.1958.sp005955
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发表时间：
1958
期刊：
影响因子：
--
通讯作者：
C. B. Taylor
中科院分区：
文献类型：
--
作者：
D. Smyth;C. B. Taylor
方法 使用的肠道制剂是 Wilson 和 Wiseman (1954) 描述的大鼠外翻小肠囊。然而，该程序经过修改，使用 30 厘米长的肠囊，而不是那些作者使用的非常短的肠囊。这些由小肠上部制备的囊悬浮在 50 ml 中。碳酸氢盐盐水（Krebs & Henseleit，1932）并在锥形烧瓶中以 90 c/min 的速率摇动。除非另有说明，碳酸氢盐盐水含有 500 mg 葡萄糖/100 ml。气相为5%二氧化碳和95%氧气，除了在厌氧实验中使用5%二氧化碳和95%氮气之外。
METHODS The intestinal preparation used was the sac of everted small intestine of the rat described by Wilson & Wiseman (1954). The procedure was, however, modified by using sacs of intestine 30 cm long instead of the very short sacs used by those authors. These sacs, prepared from the upper part of the small intestine, were suspended in 50 ml. bicarbonate saline (Krebs & Henseleit, 1932) and were shaken in conical flasks at a rate of 90 c/min. Except where otherwise stated, the bicarbonate saline contained 500 mg glucose/100 ml. The gas phase was 5% carbon dioxide and 95% oxygen except in the anaerobic experiments where 5% carbon dioxide and 95% nitrogen was used.