SEC-10 and RAB-10 coordinate basolateral recycling of clathrin-independent cargo through endosomal tubules in Caenorhabditis elegans
SEC-10 and RAB-10 coordinate basolateral recycling of clathrin-independent cargo through endosomal tubules in Caenorhabditis elegans
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DOI：
10.1073/pnas.1408327111
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发表时间：
2014-10-28
影响因子：
11.1
通讯作者：
Xu, Tao
中科院分区：
文献类型：
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作者：
Chen, Sanyou;Li, Lei;Xu, Tao
尽管在网格蛋白非依赖性内吞（CIE）途径中发现了越来越多的调节蛋白，但我们对这些蛋白的确切功能及其功能的顺序方式的了解仍然有限。在这项研究中，我们以秀丽隐杆线虫的肠道为模型，观察到一种独特的相互连接的内体小管结构，这是几种CIE货物（包括hTAC、GLUT1和DAF-4）的基侧循环所必需的。SEC-10是八聚体蛋白复合物胞囊的一个亚基。耗尽SEC-10和其他囊泡成分破坏了内体小管，使其形成各种环状结构。上位分析进一步表明，SEC-10在早期核内体和再循环核内体之间的中间阶段起作用。内体小管对Rab GTPase Rab -10的失活和微管的破坏也很敏感。综上所述，我们的数据表明SEC-10与RAB-10和微管协调形成内体管状网络，以有效地回收特定的CIE货物。
Despite the increasing number of regulatory proteins identified in clathrin-independent endocytic (CIE) pathways, our understanding of the exact functions of these proteins and the sequential manner in which they function remains limited. In this study, using the Caenorhabditis elegans intestine as a model, we observed a unique structure of interconnected endosomal tubules, which is required for the basolateral recycling of several CIE cargoes including hTAC, GLUT1, and DAF-4. SEC-10 is a subunit of the octameric protein complex exocyst. Depleting SEC-10 and several other exocyst components disrupted the endosomal tubules into various ring-like structures. An epistasis analysis further suggested that SEC-10 operates at the intermediate step between early endosomes and recycling endosomes. The endosomal tubules were also sensitive to inactivation of the Rab GTPase RAB-10 and disruption of microtubules. Taken together, our data suggest that SEC-10 coordinates with RAB-10 and microtubules to form the endosomal tubular network for efficient recycling of particular CIE cargoes.