Revealing consensus gene pathways associated with respiratory functions and disrupted by PM2.5 nitrate exposure at bulk tissue and single cell resolution
Revealing consensus gene pathways associated with respiratory functions and disrupted by PM2.5 nitrate exposure at bulk tissue and single cell resolution
复制标题
在大块组织和单细胞分辨率下揭示与呼吸功能相关并被 PM2.5 硝酸盐暴露破坏的共有基因通路
DOI：
10.1016/j.envpol.2021.116951
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发表时间：
2021-03-27
影响因子：
8.9
通讯作者：
Hao, Ke
中科院分区：
文献类型：
--
作者：
Zhang, Jushan;Cheng, Haoxiang;Hao, Ke
Background: Nitrate is a major pollutant component in ambient PM2.5. It is known that chronic exposure to PM2.5 NO3- damages respiratory functions. We aim to explore the underlying toxicological mechanism at single cell resolution.Methods: We systematically conducted exposure experiments on forty C57BL/6 mice, assessed respiratory functions, and profiled lung transcriptome. . Afterward, we estimated the cell type compositions from RNA-seq data using deconvolution analysis. The genes and pathways associated with respiratory function and dysregulated by to PM2.5 NO3- exposure were characterized at bulk-tissue and single-cell resolution.Results: PM2.5 NO3- exposure did not significantly modify the cell type composition in lung, but profoundly altered the gene expression within each cell type. At ambient concentration (22 mu g/m(3)), exposure significantly (FDR
