An explorative study of polymers for 3D printing of bioanalytical test systems
An explorative study of polymers for 3D printing of bioanalytical test systems
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DOI：
10.3233/ch-190713
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发表时间：
2020-01-01
影响因子：
2.1
通讯作者：
Roediger, Stefan
中科院分区：
文献类型：
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作者：
Jurischka, Christoph;Dinter, Franziska;Roediger, Stefan
背景技术背景：3D打印是一种用于法医、制药和生物分析应用的功能模型的制造技术，如药物输送系统、样品制备和即时检测。目的：材料的熔化行为和自发荧光对于最佳打印和产品的适用性具有决定性作用，这些都受到各种未知添加剂的影响。我们已经使用熔融层工艺从市售热塑性聚合物生产了用于生物分析应用的装置。我们通过差示扫描量热法、荧光光谱法和功能测定对其进行了表征（DNA捕获测定、细胞粘附模型、细菌粘附和生物膜形成试验）。从14种测试的有色、透明和黑色材料中，我们发现只有深黑色的丙烯腈-丁二烯-苯乙烯（ABS）和一些黑色的聚乳酸（PLA）可用于基于荧光的测定，仅在300-400 nm的短波范围内具有低的自发荧光。PLA适用于标准的生物分析目的，因为其玻璃化转变温度约为60 ℃，耐常见的实验室化学品和易于打印加工。对于温度关键的方法，如杂交反应高达90摄氏度，ABS是更好地suited.CONCLUSIONS：自体荧光本身并不是一个缺点，但也可以作为一个参考信号在测定。快速开发用于样品处理和分析的个人协议需要提供长期质量一致的材料。对于基于荧光的测定，使用商业标准物质似乎不符合这一要求。
BACKGROUND: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests.OBJECTIVE: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives.METHODS: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test).RESULTS: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60 degrees C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90 degrees C, ABS was better suited.CONCLUSIONS: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement.