Molecular cloning, characterization and positively selected sites of the glutathione S-transferase family from Locusta migratoria.
Molecular cloning, characterization and positively selected sites of the glutathione S-transferase family from Locusta migratoria.
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飞蝗谷胱甘肽S-转移酶家族的分子克隆、表征和正选位点
DOI：
10.1371/journal.pone.0114776
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zhang X;Wang J;Zhang M;Qin G;Li D;Zhu KY;Ma E;Zhang J
谷胱甘肽S-转移酶（GST）是参与内源和外源化合物代谢的多功能酶，与杀虫剂抗性有关。本研究旨在为蝗虫GST的分子特性和正选择性提供新的信息。基于转录组数据库，我们测定了28个来自飞蝗（Locusta migratoria）的胞质GST和4个微粒体GST。我们将28个胞质GST分为6类-sigma，sigma，delta，theta，omega和zeta，4个微粒体GST分为2个亚类-昆虫和MGST 3。GST的组织和阶段表达模式在mRNA水平上不同。此外，底物特异性和动力学常数的胞质GST显着不同的蛋白质水平。似然比检验的结果提供了强有力的证据，积极选择的三角洲类。贝叶斯经验贝叶斯分析的结果确定δ类中的4个氨基酸位点为正选择位点。这些位点位于蛋白质表面。我们的研究结果将有助于阐明昆虫GST超家族的分子特征和进化方面。
Glutathione S-transferases (GSTs) are multifunctional enzymes that are involved in the metabolism of endogenous and exogenous compounds and are related to insecticide resistance. The purpose of this study was to provide new information on the molecular characteristics and the positive selection of locust GSTs. Based on the transcriptome database, we sequenced 28 cytosolic GSTs and 4 microsomal GSTs from the migratory locust (Locusta migratoria). We assigned the 28 cytosolic GSTs into 6 classes—sigma, epsilon, delta, theta, omega and zeta, and the 4 microsomal GSTs into 2 subclasses—insect and MGST3. The tissue- and stage-expression patterns of the GSTs differed at the mRNA level. Further, the substrate specificities and kinetic constants of the cytosolic GSTs differed markedly at the protein level. The results of likelihood ratio tests provided strong evidence for positive selection in the delta class. The result of Bayes Empirical Bayes analysis identified 4 amino acid sites in the delta class as positive selection sites. These sites were located on the protein surface. Our findings will facilitate the elucidation of the molecular characteristics and evolutionary aspects of insect GST superfamily.
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