Age- and Sex-Associated Differences in the Glycopatterns of Human Salivary Glycoproteins and Their Roles against Influenza A Virus
Age- and Sex-Associated Differences in the Glycopatterns of Human Salivary Glycoproteins and Their Roles against Influenza A Virus
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DOI：
10.1021/pr400096w
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发表时间：
2013-06-01
影响因子：
4.4
通讯作者：
Li, Zheng
中科院分区：
文献类型：
--
作者：
Qin, Yannan;Zhong, Yaogang;Li, Zheng
近年来的研究表明，人类唾液中某些糖蛋白的表达随着年龄的增长而增加或减少;同时，人类唾液可以抑制病毒感染，阻止病毒传播。然而，很少有人知道的年龄和性别相关的差异，人类唾液糖蛋白的糖蛋白和他们的显着作用，对甲型流感病毒（IVA）。在这里，我们调查了180个健康的唾液样本分为6个年龄/性别组，使用凝集素微阵列和制作唾液微阵列，以验证在个人唾液样本中的糖蛋白的末端碳水化合物部分的糖蛋白的糖蛋白。此外，我们评估了唾液对两种甲型流感（H9 N2）病毒株的抑制和中和活性。我们发现七种凝集素（例如，MAL-II和SNA）在女性和男性中均显示出显著的年龄差异，并且七种凝集素（例如，WFA和STL）在儿童、成人和老年人中显示出显著的性别差异。有趣的是，我们观察到老年人对IVA具有最强的抵抗力，部分原因是他们的唾液中存在更多的末端α 2-3/6连接的唾液酸残基，这些残基与流感病毒血凝结合。我们的结论是，年龄和性别相关的差异，人类唾液糖蛋白的糖代谢可能提供关键的信息，以帮助了解一些年龄相关的疾病和生理现象。
Recent studies have elucidated that expression of certain glycoproteins in human saliva is increased or decreased according to age; meanwhile, human saliva may inhibit viral infection and prevent viral transmission. However, little is known about the age- and sex-associated differences in the glycopatterns of human salivary glycoproteins and their significant roles against influenza A virus (IVA). Here, we investigate the glycopatterns of human salivary glycoproteins with 180 healthy saliva samples divided into six age/sex groups using lectin microarrays and fabricate saliva microarrays to validate the terminal carbohydrate moieties of glycoproteins in individual saliva samples. Furthermore, we assess the inhibiting and neutralizing activity of saliva against two strains of influenza A (H9N2) virus. We find that seven lectins (e.g., MAL-II and SNA) show significant age differences in both females and males, and seven lectins (e.g., WFA and STL) show significant sex differences in children, adults and elderly people. Interestingly, we observe that elderly individuals have strongest resistance to IVA partly by presenting more terminal alpha 2-3/6-linked sialic acid residues in their saliva, which bind with the influenza viral hemagglutinations. We conclude that age- and sex-associated differences in the glycopatterns of human salivary glycoproteins may provide pivotal information to help understand some age related diseases and physiological phenomena.