Extracellular pH is a biomarker enabling detection of breast cancer and liver cancer using CEST MRI
Extracellular pH is a biomarker enabling detection of breast cancer and liver cancer using CEST MRI
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细胞外 pH 值是一种生物标志物，可使用 CEST MRI 检测乳腺癌和肝癌
DOI：
10.18632/oncotarget.17404
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发表时间：
2017-07-11
期刊：
影响因子：
--
通讯作者：
Wu, Renhua
中科院分区：
文献类型：
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作者：
Chen, Miaomiao;Chen, Chaoying;Wu, Renhua
细胞外 pH (pHe) 降低与肿瘤生长、侵袭、转移和化疗耐药相关，可通过化学交换饱和转移 (CEST) 磁共振成像 (MRI) 检测到。在这里，我们证明了碘佛醇 CEST MRI 可用于实现肝癌微环境的 pHe 绘图。在体外研究中，我们首先探讨了碘佛醇信号是否具有pH依赖性，并通过比例法计算了碘佛醇的CEST效应与pH值（6.0至7.8范围内）之间的函数方程。然后，我们通过在 MMTV-Erbb2 转基因小鼠乳腺癌模型中应用 pHe 成像来验证该技术和体内方程的可行性，该模型经常用于 CEST pHe 研究。此外，通过体内碘佛醇 CEST MRI，我们能够在 McA-RH7777 大鼠肝癌模型中绘制相对 pHe 并区分肿瘤和正常组织。这表明 pHe 可能是人类肝癌的有用生物标志物。
Extracellular pH (pHe) decrease is associated with tumor growth, invasion, metastasis, and chemoresistance, which can be detected by chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI). Here, we demonstrated that ioversol CEST MRI can be exploited to achieve pHe mapping of the liver cancer microenvironment. In in vitro studies, we firstly explored whether ioversol signal is pH-dependent, and calculated the function equation between the CEST effects of ioversol and pH values, in the range of 6.0 to 7.8, by a ratiometric method. Then we verified the feasibility of this technique and the equation in vivo by applying pHe imaging in an MMTV-Erbb2 transgenic mouse breast cancer model, which is often used in CEST pHe studies. Furthermore, in vivo ioversol CEST MRI, we were able to map relative pHe and differentiate between tumor and normal tissue in a McA-RH7777 rat hepatoma model. This suggests pHe may be a useful biomarker for human liver cancer.