Monopolar protrusive activity:: A new morphogenic cell behavior in the neural plate dependent on vertical interactions with the mesoderm in Xenopus
Monopolar protrusive activity:: A new morphogenic cell behavior in the neural plate dependent on vertical interactions with the mesoderm in Xenopus
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DOI：
10.1006/dbio.2000.9746
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发表时间：
2000-08-01
影响因子：
2.7
通讯作者：
Keller, R
中科院分区：
文献类型：
--
作者：
Elul, T;Keller, R
我们通过视频记录附着有退化中胚层的深层神经组织和单独的深层神经组织的外植体，比较了在存在和不存在来自中胚层的持续垂直信号的情况下驱动爪蟾神经板会聚延伸的形态发生细胞行为的类型和模式。在深层神经中胚层外植体中，神经板细胞表达单极内侧定向运动，而诺托板细胞表达随机定向运动，这是两种新的形态发生细胞行为。相反，在深层神经外植体（无托板）中，所有细胞都表现出双极中外侧细胞运动。中胚层上的深层神经和深层神经外植体在内外侧细胞嵌入过程中相邻交换的程度也不同。在深层神经中胚层外植体中，细胞插入保守，而在深层神经外植体中，细胞插入更加混杂。最后，在中胚层上的深层神经和深层神经外植体中，形态发生细胞行为在前到后和从外侧到内侧的进展中有所不同。然而，在深层神经-中胚层外植体中，形态发生行为首先沿前后轴间隔分化，而在深层神经外植体中，形态发生行为从组织前端向后连续分化。这些结果描述了驱动神经会聚延伸的新形态发生细胞行为，并定义了中胚层信号在原肠胚形成晚期和之后在这些细胞行为模式化中的作用。 (C) 2000 年学术出版社。
We compared the type and patterning of morphogenic cell behaviors driving convergent extension of the Xenopus neural plate in the presence and absence of persistent vertical signals from the mesoderm by videorecording explants of deep neural tissue with involuted mesoderm attached and of deep neural tissue alone. In deep neural-over-mesoderm explants, neural plate cells express monopolar medially directed motility and notoplate cells express randomly oriented motility, two new morphogenic cell behaviors. In contrast, in deep neural explants (without notoplate), all cells express bipolar mediolateral cell motility. Deep neural-over-mesoderm and deep neural explants also differ in degree of neighbor exchange during mediolateral cell intercalation. In deep neural-over-mesoderm explants, cells intercalate conservatively, whereas in deep neural explants cells intercalate more promiscuously. Last, in both deep neural-over-mesoderm and deep neural explants, morphogenic cell behaviors differentiate in an anterior-to-posterior and lateral-to-medial progression. However, in deep neural-over-mesoderm explants, morphogenic behaviors first differentiate in intervals along the anteroposterior axis, whereas in deep neural explants, morphogenic behaviors differentiate continuously from the anterior end of the tissue posteriorly. These results describe new morphogenic cell behaviors driving neural convergent extension and also define roles for signals from the mesoderm, up to and beyond late gastrulation, in patterning these cell behaviors. (C) 2000 Academic Press.