Carbon dots obtained using hydrothermal treatment of formaldehyde. Cell imaging in vitro
Carbon dots obtained using hydrothermal treatment of formaldehyde. Cell imaging in vitro
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DOI：
10.1039/c4nr01585a
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发表时间：
2014-08-07
期刊：
影响因子：
6.7
通讯作者：
Casado, J.
中科院分区：
文献类型：
--
作者：
Algarra, M.;Perez-Martin, M.;Casado, J.
高度光致膨胀的碳量子点已经通过在180 ℃下对甲醛进行水热处理而在一个步骤中制备。它们在紫外光照射下显示绿色荧光，发射光谱中心在440 nm。当合成过程持续1-7天时，荧光寿命在0.7和2.70 ns之间。纳米颗粒的TEM图像显示出均匀的尺寸/形状分布。当热处理过程进行很长时间（30天）时，发生聚集体的形成。使用H-1和C-13-NMR、拉曼和FTIR光谱技术和XPS进一步分析碳量子点。通过使用小鼠MC 3 T3-E1前成骨细胞作为模型进行纳米颗粒的细胞成像。纳米颗粒被选择性地定位在细胞质中而不进一步功能化，并且可以通过细胞吞噬作用来实现，使得这些纳米颗粒的荧光可以用于体外活细胞成像。
Highly phototuminescent carbon dots have been prepared in a one step procedure by hydrothermal treatment of formaldehyde at 180 degrees C. They show green fluorescence under UV light exposure and emission spectra are centered at 440 nm. Fluorescence lifetimes comprise between 0.7 and 2.70 ns, when the synthesis process lasted for 1-7 days. TEM images of nanoparticles showed a homogeneous size/shape distribution. When the thermal treatment process was carried out for a long time (30 days) formation of aggregates occurred. Carbon dots were further analyzed using H-1 and C-13-NMR, Raman and FTIR spectroscopy techniques and XPS. Cell imaging of nanopartictes was carried out by using mouse MC3T3-E1 pre-osteoblasts as a model. The nanoparticles were selectively localized in the cytoplasm without further functionalization and could be realized by cellular phagocytosis, so that the fluorescence of these can be used for live cell imaging in vitro.