Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research.
Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research.
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DOI：
10.1016/j.jid.2016.01.005
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Kong HH
中科院分区：
文献类型：
--
作者：
Jo JH;Kennedy EA;Kong HH
Skin serves as a protective barrier and also harbors numerous microorganisms collectively comprising the skin microbiome. As a result of recent advances in sequencing (Next-Generation Sequencing, NGS), our understanding of microbial communities on skin has advanced substantially. In particular, the 16S ribosomal RNA (rRNA) gene sequencing technique has played an important role in efforts to identify the global communities of bacteria in healthy individuals and patients with various disorders in multiple topographical regions over the skin surface. Here, we describe basic principles, study design, and a workflow of 16S rRNA gene sequencing methodology, primarily for investigators who are not familiar with this approach. This article will also discuss some applications and challenges of 16S rRNA sequencing as well as directions for future development.
