Modulation of histamine release from canine fundic mucosal mast cells.
Modulation of histamine release from canine fundic mucosal mast cells.
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调节犬胃底粘膜肥大细胞释放组胺。
DOI：
10.1152/ajpgi.1988.254.1.g40
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Beaven,MA
中科院分区：
文献类型：
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作者：
Soll,AH;Toomey,M;Culp,D;Shanahan,F;Beaven,MA
为了研究组胺释放的控制，我们开发了培养胃底粘膜肥大细胞的技术。经过酶分散、淘洗富集和过夜悬浮培养后，肥大细胞占存在细胞的30%。通过放射酶法测定，凝集素伴刀豆球蛋白 A (Con A) 刺激组胺释放到培养基中。豚草抗原在抗血清致敏培养物中释放组胺。腺苷可增强Con A诱导的组胺释放，但单独使用腺苷则无活性。腺苷类似物的相对效力与腺苷 A1 受体位点的相互作用一致。钙离子载体 A23187 (0.1-1 microM) 也诱导组胺释放。激活蛋白激酶 C 的佛波酯（例如佛波醇 12-肉豆蔻酸酯 13-乙酸酯）不会释放组胺，但在添加到低浓度的 A23187 中时会增强释放。相反，无活性的佛波醇，例如4α-佛波醇12,13-二癸酸酯，未能增强A23187诱导的释放。对犬肝肥大细胞的平行研究产生了可比较的结果。我们得出结论，犬胃底肥大细胞拥有免疫球蛋白 E 和腺苷受体。我们的数据与胞质钙和蛋白激酶 C 激活协同作用刺激胃底肥大细胞的增加一致。
To study the control of histamine release, we developed techniques for culturing fundic mucosal mast cells. After enzyme dispersion, enrichment by elutriation, and overnight suspension culture, mast cells accounted for 30% of the cells present. Histamine release into the medium, measured by radioenzymatic assay, was stimulated by the lectin concanavalin A (Con A). Ragweed antigen released histamine in antisera-sensitized cultures. Con A-induced histamine release was enhanced by adenosine, but adenosine alone was inactive. The relative potency of adenosine analogues was consistent with interaction at an adenosine A1-receptor site. The calcium ionophore A23187 (0.1-1 microM) also induced histamine release. Phorbol esters that activate protein kinase C, such as phorbol 12-myristate 13-acetate, did not release histamine but enhanced release when added to low concentrations of A23187. In contrast, inactive phorbols, such as 4 alpha-phorbol 12,13-didecanoate, failed to enhance A23187-induced release. Parallel studies with canine hepatic mast cells yielded comparable results. We conclude that canine fundic mast cells possess receptors for immunoglobulin E and adenosine. Our data are consistent with increases in cytosolic calcium and protein kinase C activation working synergistically to stimulate fundic mast cells.