Acquisition of LURE-Binding Activity at the Pollen Tube Tip of Torenia fournieri
Acquisition of LURE-Binding Activity at the Pollen Tube Tip of Torenia fournieri
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DOI：
10.1093/mp/sst050
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发表时间：
2013-07-01
期刊：
影响因子：
27.5
通讯作者：
Higashiyama, Tetsuya
中科院分区：
文献类型：
--
作者：
Okuda, Satohiro;Suzuki, Takamasa;Higashiyama, Tetsuya
我们发现，在蓝猪耳的花粉管引导，结合的引诱肽LURE在管尖和响应能力LURE不同的雌蕊组织控制。同时也提供了花粉管的转录组和蛋白质组数据。花粉管的引导是由与雌性组织的多种复杂的相互作用控制的。在这里，我们表明，蓝猪耳的花粉管调节的花柱组织在长度依赖性的方式接收和响应引诱剂LURE肽分泌助细胞。我们开发了一种免疫染色方法来可视化结合在花粉管尖端区域的质膜上的LURE肽。使用这种方法，我们发现LURE肽特异性结合到通过切割花柱生长的花粉管上。这些肽还结合到通过较短花柱生长的花粉管上，这些花粉管不能对这些肽做出反应。这些观察结果表明，LURE肽接收能力的获得和完全能力的获得可能是可分离的过程。RNA-Seq结果表明，花粉管的转录谱受花柱长度和培养时间的影响，与结合活性和LURE响应能力的生理变化一致。从猪耳属花粉管的从头RNA-Seq产生的数据库被证明是有用的，通过质谱鉴定花粉管蛋白。我们的研究为蛋白质鉴定提供了一个有效的平台，以了解花粉管引导。
We show that, in the pollen tube guidance of Torenia, binding of an attractant peptide LURE at the tube tip and response competency to LURE are differently controlled by the pistil tissue. Transcriptome and proteome data of pollen tubes are also provided.Pollen tube guidance is controlled by multiple complex interactions with the female tissues. Here, we show that pollen tubes of Torenia fournieri are regulated by a stylar tissue in a length-dependent manner to receive and respond to attractant LURE peptides secreted from synergid cells. We developed an immunostaining method to visualize LURE peptides bound at the plasma membrane of the tip region of the pollen tube. Using this method, we found that LURE peptides bound specifically to pollen tubes growing through a cut style. The peptides also bound to pollen tubes growing through a shorter style, which were not competent to respond to these peptides. These observations suggested a possibility that acquisition of the LURE peptide reception ability and acquisition of full competency are separable processes. RNA-Seq suggested that the transcription profile of pollen tubes was affected by both the length of the style and the cultivation period, consistently with physiological changes in binding activity and LURE response ability. The database generated from de novo RNA-Seq of Torenia pollen tubes was shown to be useful to identify pollen tube proteins by mass spectrometry. Our studies provide insight and an effective platform for protein identification to understand pollen tube guidance.