A mouse knockout library for secreted and transmembrane proteins
A mouse knockout library for secreted and transmembrane proteins
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DOI：
10.1038/nbt.1644
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发表时间：
2010-07-01
影响因子：
46.9
通讯作者：
de Sauvage, Frederic J.
中科院分区：
文献类型：
--
作者：
Tang, Tracy;Li, Li;de Sauvage, Frederic J.
大量的基因敲除生物体有助于阐明基因功能。在这里，我们使用逆转录病毒插入或同源重组来扰乱小鼠472个编码分泌和膜蛋白的基因，为研究这一重要类别的药物靶点的很大一部分提供了资源。基因敲除的小鼠接受了系统的表型筛选，旨在揭示胚胎发育、新陈代谢、免疫系统、神经系统和心血管系统的变化。大多数基因敲除品系至少在其中一个治疗区域表现出改变的表型。据我们所知，以前还没有对由基因特异性方法产生的大量小鼠突变进行全面的表型评估。
Large collections of knockout organisms facilitate the elucidation of gene functions. Here we used retroviral insertion or homologous recombination to disrupt 472 genes encoding secreted and membrane proteins in mice, providing a resource for studying a large fraction of this important class of drug target. The knockout mice were subjected to a systematic phenotypic screen designed to uncover alterations in embryonic development, metabolism, the immune system, the nervous system and the cardiovascular system. The majority of knockout lines exhibited altered phenotypes in at least one of these therapeutic areas. To our knowledge, a comprehensive phenotypic assessment of a large number of mouse mutants generated by a gene-specific approach has not been described previously.