Analysis of α- and β-tubulin genes of Bombyx mori using an EST database
Analysis of α- and β-tubulin genes of Bombyx mori using an EST database
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DOI：
10.1016/s0965-1748(02)00184-4
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发表时间：
2003-01-01
影响因子：
3.8
通讯作者：
Mita, K
中科院分区：
文献类型：
--
作者：
Kawasaki, H;Sugaya, K;Mita, K
微管蛋白是多蛋白家族中最广泛的一类，并且众所周知，微管蛋白具有两种不同的亚基，α-微管蛋白和β-微管蛋白。在家蚕基因组分析过程中，我们从家蚕不同发育阶段的组织器官cDNA文库中随机选取克隆，进行大规模测序，构建了EST数据库。利用该EST数据库，我们鉴定了四种类型的β-微管蛋白基因和三种类型的α-微管蛋白基因。基于推导的氨基酸序列的分析，我们已经确定了微管蛋白之间的系统发育关系的果蝇和黑腹果蝇以及其他两个蛾类，表明每个微管蛋白至少分为三个不同的亚科：一个普遍表达的，发育调节的和睾丸特异性的。(C)2002爱思唯尔科技有限公司版权所有。
Tubulin is one of the most widespread classes of multiprotein families and is well known to construct microtubules with two different subunits, alpha- and beta-tubulin. In the course of genome analysis of Bombyx mori, we have constructed an EST database by large-scale sequencing of clones that were randomly selected from cDNA libraries of various tissues and organs belonging to different developmental stages. Using this EST database, we have identified four types of beta-tubulin gene and three types of alpha-tubulin gene. Based on the analysis of deduced amino acid sequences, we have determined the phylogenetic relationships of tubulins between Bombyx and Drosophila melanogaster as well as two other moth species, suggesting that each tubulin is classified into at least three distinct subfamilies: a ubiquitously expressed one, a developmentally regulated one and a testis specific one. (C) 2002 Elsevier Science Ltd. All rights reserved.