Chromosome-level genome assemblies of two cotton-melon aphid Aphis gossypii biotypes unveil mechanisms of host adaption
Chromosome-level genome assemblies of two cotton-melon aphid Aphis gossypii biotypes unveil mechanisms of host adaption
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DOI：
10.1111/1755-0998.13521
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发表时间：
2021-10-11
影响因子：
7.7
通讯作者：
Yang，Yizhong
中科院分区：
文献类型：
--
作者：
Zhang，Shuai;Gao，Xueke;Yang，Yizhong
棉蚜（Aphis gossypii）是一种吸液昆虫，被认为是一种严重的全球性害虫。该物种分布在很大的地理范围内，并使用各种各样的宿主，其中一些种群专门攻击不同的植物物种。在这里，我们提供了棉花专家群体（Hap 1）和葫芦科专家群体（Hap 3）的新染色体水平的基因组组装。我们通过使用第三代测序平台的组合实现了这一目标，即Illumina和Hi-C测序技术。我们能够分别将总共334.89 Mb（支架N50为89.13 Mb）和359.95 Mb（支架N50为68.88 Mb）锚定到Hap 1和Hap 3的四条染色体上。此外，我们的研究结果表明，Hap 3的X染色体（113.01 Mb）明显长于Hap 1的X染色体（100.26 Mb），在蚜虫物种之间具有高度的序列保守性。我们还报告了Hap 1和Hap 3之间蛋白质编码基因和重复序列数量的变化。特别是，嗅觉和味觉受体基因在棉曲霉中经历了高水平的基因复制和扩增事件，包括Hap 1和Hap 3之间。此外，我们确定了两个谷胱甘肽S-转移酶基因，在Hap 3中经历了单基因复制，以及影响Hap 1和Hap 3之间细胞色素P450单加氧酶的串联复制和倒位事件，所有这些都包括CYP 3家族。研究结果揭示了棉蚜两个特化种群基因组组成的差异，为棉蚜种群进化、寄主适应和抗药性研究提供了有益的参考。
The cotton‐melon aphidAphis gossypiiis a sap‐sucking insect that is considered a serious global pest. The species is distributed over a large geographical range and uses a wide variety of hosts, with some populations being specialized to attack different plant species. Here, we providede novochromosome‐level genome assemblies of a cotton specialist population (Hap1) and a cucurbit specialist population (Hap3). We achieved this by using a combination of third‐generation sequencing platforms, namely Illumina and Hi‐C sequencing technologies. We were able to anchor a total of 334.89 Mb (scaffold N50 of 89.13 Mb) and 359.95 Mb (scaffold N50 of 68.88 Mb) to four chromosomes for Hap1 and Hap3, respectively. Moreover, our results showed that the X‐chromosome of Hap3 (113.01 Mb) was significantly longer than that of Hap1 (100.26 Mb), with a high level of sequence conservation between the aphid species. We also report variation in the number of protein‐coding genes and repeat sequences between Hap1 and Hap3. In particular, olfactory and gustatory receptor genes underwent a high level of gene duplication and expansion events inA.gossypii, including between Hap1 and Hap3. Moreover, we identified two glutathioneS‐transferase genes which underwent single gene duplications in Hap3, and tandem duplication and inversion events affecting the cytochrome P450 monooxygenase between Hap1 and Hap3, all of which include the CYP3 family. Our results illustrate the variance in the genomic composition of two specializedA.gossypiipopulations and provide a helpful resource for the study of aphid population evolution, host adaption and insecticide resistance.