Role of circulating white blood cells in the enhancement of antigen-induced bronchoconstriction after intravascular complement activation with cobra venom factor.
Role of circulating white blood cells in the enhancement of antigen-induced bronchoconstriction after intravascular complement activation with cobra venom factor.
复制标题
眼镜蛇毒因子激活血管内补体后循环白细胞在增强抗原诱导的支气管收缩中的作用。
DOI：
10.1111/j.1749-6632.1991.tb37992.x
复制
发表时间：
1991
影响因子：
5.2
通讯作者：
Fraser,DG
中科院分区：
文献类型：
--
作者：
Regal,JF;Fraser,DG
在离体心脏中的研究已经证明，补体激活在心脏过敏性事件中起放大系统的作用。“我们以前的研究表明，如果动物首先用眼镜蛇毒因子（CVF）预处理以激活补体系统，那么抗原诱导的豚鼠支气管收缩会明显更严重。2这些研究结果表明，补体系统激活可能会加剧过敏性支气管收缩，本研究的目的是确定CVF血管内补体激活对抗原诱导的支气管收缩的增强是否依赖于循环白色血细胞或血小板的存在。如前所述，在戊巴比妥麻醉的机械通气豚鼠中监测气管气流和经肺动脉压。3在用卵清蛋白IgG抗体被动致敏的动物中，计算肺阻力和动态肺顺应性作为卵清蛋白iv激发后的支气管收缩的量度。使用通过先前公开的方法制备的特异性抗血清，使豚鼠的循环白色血细胞（主要是粒细胞）和/或血小板耗尽。抗血清处理对循环细胞群的有效性显示在图1中。用抗白细胞血清治疗可显著减少循环中粒细胞的数量，对循环中血小板和单核细胞的数量影响较小，而用抗白细胞和抗血小板血清联合治疗仅减少粒细胞和血小板的数量，而不显著改变单核细胞的数量。消耗后，用CVF或溶剂预处理动物，并监测对抗原的反应。如图2所示，CVF处理具有正常数量的循环白色血细胞和血小板的豚鼠（对照）导致抗原诱导的支气管收缩的显著增强，如先前报道的。2如果动物首先耗尽循环血小板，CVF增强仍然发生（数据未显示）。然而，如果动物的循环白色血细胞，主要是粒细胞被耗尽，CVF的作用被逆转，抗原诱导的支气管收缩与对照相似（图2）。COM-
Studies in the isolated heart have demonstrated that complement activation functions as an amplification system in the cardiac anaphylactic event.'Our previous studies have indicated that antigen-induced bronchoconstriction in the guinea pig is significantly more severe if animals are first pretreated with cobra venom factor (CVF) to activate the complement system. 2 The results of these studies suggest that allergic bronchoconstriction may be exacerbated by complement system activation.The purpose of the present study was to determine if the enhancement of antigen-induced bronchoconstriction by intravascular complement activation with CVF was dependent on the presence of circulating white blood cells or platelets. Tracheal airflow and transpulmonary pressure were monitored in pentobarbital anesthetized, mechanically ventilated guinea pigs as previously described. 3 Pulmonary resistance and dynamic lung compliance were calculated as a measure of bronchoconstriction after iv challenge with ovalbumin in animals passively sensitized with IgG antibody to ovalbumin. Guinea pigs were depleted of circulating white blood cells (primarily granulocytes) and/or platelets using specific antisera prepared by previously published method^.^ The effectiveness of the antisera treatment on circulating cell populations is shown in FIGURE 1. Treatment with anti-white blood cell serum drastically reduced the number of circulat-ing granulocytes with a minor effect on the numbers of circulating platelets and mononuclear cells, whereas combined treatment with both anti-white blood cell and anti-platelet serum reduced only the numbers of granulocytes and platelets without significantly altering the numbers of mononuclear cells. After depletion, animals were pretreated with CVF or vehicle and the response to antigen monitored. As shown in FIGURE 2, CVF treatment of a guinea pig with normal numbers of circulating white blood cells and platelets (control) resulted in a significant enhancement of the antigen-induced bronchoconstriction, as previously reported. 2 This CVF enhancement still occurred if animals were first depleted of circulating platelets (data not shown). However, if animals were depleted of circulating white blood cells, primarily granulocytes, the effect of CVF was reversed and the antigen-induced bronchoconstriction was similar to control (FIG. 2). Com-