THEILERS VIRUS-INFECTION IN MICE - UNUSUAL BIPHASIC DISEASE PROCESS LEADING TO DEMYELINATION
THEILERS VIRUS-INFECTION IN MICE - UNUSUAL BIPHASIC DISEASE PROCESS LEADING TO DEMYELINATION
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DOI：
10.1128/iai.11.5.1147-1155.1975
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
LIPTON, HL
中科院分区：
文献类型：
--
作者：
LIPTON, HL
一种不寻常的双相中枢神经系统疾病发生在3周大的瑞士近交种小鼠脑内接种泰勒氏小鼠脑脊髓炎病毒DA株后。感染后9至20天，86%的小鼠瘫痪，大约一半的小鼠存活。在此期间，丘脑、脑干和脊髓可见神经元坏死和小胶质细胞增生。脊髓内有病毒生长的初始阶段，随后在较低浓度下持续感染。在感染早期，免疫荧光染色在神经元细胞质中很容易发现病毒抗原，而在30天后，含有病毒抗原的细胞很少，仅存在于脊髓白质中。在1到5个月之间，脊髓轻脑膜和白质中出现了强烈的单核炎症细胞病变，与此同时，一些存活的小鼠出现了轻微的步态障碍，并且在炎症区域发现了斑块性脱髓鞘。急性灰质病理似乎是直接病毒裂解作用的结果。虽然以脱髓鞘为终点的晚期白质病变可能代表了一种类似于某些小核糖核酸载体培养系统中存在的细胞杀细胞感染，但病毒诱导的免疫病理过程值得进一步研究。
An unusual biphasic central nervous system disease developed in 3-week-old Swiss outbred mice after intracerebral inoculation of the DA strain of Theiler's murine encephalomyelitis virus. Nine to 20 days postinfection 86% of mice became paralyzed, and approximately one-half of these animals survived. During this period neuronal necrosis and microglial proliferation were seen in thalamus, brainstem, and spinal cord. There was an initial phase of virus growth in spinal cord followed by persistent infection at a lower concentration. Virus antigen was readily found in the cytoplasm of neurons by immunofluorescent staining early in the course of infection, whereas after 30 days there was a paucity of cells containing virus antigen which were present only in the spinal cord white matter. Between 1 and 5 months, an intense mononuclear inflammatory cell lesion evolved in the spinal cord leptomeninges and white matter, which coincided with a mild gait disturbance in some surviving mice, and patchy demyelination was found in areas of inflammation. The acute gray matter pathology would appear to be the result of direct virus lytic effect. Although the late white matter lesion culminating in demyelination probably represents a cytocidal infection similar to the situation that exists in certain picornavirus carrier culture systems, a virus-induced immunopathological process merits further study.