Investigation of metabolic changes in irradiated rat brain tissue by means of 1H NMR in vitro relaxation study
Investigation of metabolic changes in irradiated rat brain tissue by means of 1H NMR in vitro relaxation study
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DOI：
10.1016/j.ssnmr.2003.03.013
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发表时间：
2004-01-01
影响因子：
3.2
通讯作者：
Matlas, B
中科院分区：
文献类型：
--
作者：
Sokól, M;Przybyszewski, WM;Matlas, B
本文用高分辨H-1 NMR研究了离体照射对脑代谢物浓度和弛豫行为的影响。对大鼠脑进行了研究，以2戈伊的剂量照射20戈伊。使用标准程序获得大鼠脑的HClO 4提取物。使用Varian Inova-300 NMR光谱仪对浸提液的D2 O溶液进行H-1 NMR研究。代谢物信号的积分强度在辐照过程中和辐照后都发生了变化，这些变化伴随着T1弛豫时间的变化。N-乙酰天冬氨酸，甘油磷酸胆碱，磷酸胆碱，胆碱，肌酸和磷酸肌酸，肌醇和牛磺酸作为辐射损伤的潜在标志物进行了分析。(C)2003年爱思唯尔公司All rights reserved.
The effect of irradiation on concentrations and relaxation behaviour of brain metabolites was studied by means of high-resolution H-1 NMR in vitro. The studies were performed on rat brains irradiated with the doses of 20 Gy applied in fractions of 2 Gy. Standard procedures were used to obtain HClO4 extracts of rat brains. The H-1 NMR studies of the extracts solutions in D2O were performed using a Varian Inova-300 NMR spectrometer. The integral intensities of the metabolite signals were found to change during the irradiation cycle and after it. These changes are accompanied by the variations in the T1 relaxation times. N-acetylaspartate, glycerophosphocholine, phosphocholine, choline, creatine and phosphocreatine, myoinositol and taurine were analysed as potential markers of irradiation injury. (C) 2003 Elsevier Inc. All rights reserved.