Molecular characterization and analysis of the association of growth hormone 1 gene with growth traits in Chinese indigenous yak (Bos grunniens)
Molecular characterization and analysis of the association of growth hormone 1 gene with growth traits in Chinese indigenous yak (Bos grunniens)
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DOI：
10.1007/s11250-021-02671-w
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发表时间：
2021-03
影响因子：
1.7
通讯作者：
L. Gui;S. A. Raza;Yong-gang Sun;A. Sabek;Sayed Qaisar Abbas;Mujahid Ali Shah;Rajwali Khan;S. Abdelnour
中科院分区：
文献类型：
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作者：
L. Gui;S. A. Raza;Yong-gang Sun;A. Sabek;Sayed Qaisar Abbas;Mujahid Ali Shah;Rajwali Khan;S. Abdelnour
本研究旨在探讨生长激素1（GH 1）基因多态性对中国牦牛生长性状的影响。采用聚合酶链反应-限制性片段长度多态性（PCR-PFLP）方法，在牦牛GH 1基因第4外显子上发现了一个新的单核苷酸多态性（SNP），命名为g.1721G>A。经卡方（χ2）检验，g.1721G>A等位基因频率符合Hardy-Weinberg平衡（HWE）（P< 0.05）。该SNP与几个生长性状间存在显著相关（P< 0.01或P < 0.05），其中GG基因型的相关性最好。本研究表明，该SNP位点可作为牦牛生长性状改良的遗传标记。
This study aimed to investigate the effects of polymorphisms in growth hormone 1 (GH1) gene on the growth traits in Chinese indigenous yak. Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP) approach, one novel single-nucleotide polymorphism (SNP), termed as g.1721G>A, was identified in the exon 4 ofGH1gene in 423 individuals of yak population. Based on the chi-square (χ2) test, the frequencies of g.1721G>A alleles agreed with Hardy-Weinberg equilibrium (HWE) (P< 0.05). A significant association was observed between this SNP and several growth traits (P< 0.01 orP< 0.05), in which the genotype GG exhibited the best values. The present study suggested that the identified SNP was a useful genetic marker for the improvement of growth traits in Chinese indigenous yak.