A high‐performance neutron diffractometer for biological crystallography (BIX‐3)
A high‐performance neutron diffractometer for biological crystallography (BIX‐3)
复制标题
用于生物晶体学的高性能中子衍射仪（BIX-3）
DOI：
10.1107/s0021889801017745
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发表时间：
2002
影响因子：
6.1
通讯作者：
N. Niimura
中科院分区：
文献类型：
--
作者：
I. Tanaka;K. Kurihara;T. Chatake;N. Niimura
为了测定生物大分子中氢原子的位置，在日本原子能研究所（JAERI）的JRR-3M上建造了一台用于生物晶体学的高性能中子衍射仪（BIX-3）。它使用了几项最新的技术创新，如中子成像板和作者开发的弹性弯曲硅单色器。这些使得能够实现衍射仪的紧凑的垂直布置。在大约一个月的时间里，从蛋白质rubredoxin和肌红蛋白中收集了衍射数据，分辨率为1.5 A。 这些数据足以以高精度识别氢原子。采用晶体步进扫描法测量布喇格衍射强度，信噪比比劳厄法好得多。这表明BIX-3是当今世界上性能最好的中子蛋白质晶体学机器之一。
A high-performance neutron diffractometer for biological crystallography (BIX-3) has been constructed at JRR-3M in the Japan Atomic Energy Research Institute (JAERI) in order to determine the hydrogen-atom positions in biological macromolecules. It uses several recent technical innovations, such as a neutron imaging plate and an elastically bent silicon monochromator developed by the authors. These have made it possible to realise a compact vertical arrangement of the diffractometer. Diffraction data have been collected from the proteins rubredoxin and myoglobin in about one month, to a resolution of 1.5 A. The data were good enough to identify the hydrogen atoms with high accuracy. By adopting a crystal-step scan method for measuring Bragg diffraction intensities, the signal-to-noise ratio was much better than that of the Laue method. This shows that BIX-3 is one of the best-performing machines for neutron protein crystallography in the world today.