The role of zinc ions in the transformation of lymphocytes by phytohaemagglutinin.
The role of zinc ions in the transformation of lymphocytes by phytohaemagglutinin.
复制标题
锌离子在植物血凝素转化淋巴细胞中的作用。
作者：
J. Chesters
1. [(3)H]胸苷掺入 DNA 会被 EDTA 和二亚乙基三胺-NNN'N''N''-五乙酸盐抑制，但不会被次氮基三乙酸盐抑制，即使 Ca(2+) 的浓度超过螯合剂浓度的两倍也是如此。 2. EDTA 引起的抑制作用最有效地被 Zn(2+) 逆转，但也被 Cd(2+) 逆转。单独添加Co(2+)、Cu(2+)、Fe(3+)、Mn(2+) 或Ni(2+) 时，EDTA 抑制系统的激活作用非常小（如果有的话）。 3. 在 EDTA 存在下，将 Fe(3+) 添加到 Zn(2+) 激活的淋巴细胞中，显着增加胸苷掺入。 Cd(2+) 的添加阻止了高 Zn(2+) 浓度下发生的掺入抑制。 4.如果在植物血凝素之后添加EDTA超过15小时，则对掺入的抑制小于零时添加所获得的抑制。如果在 EDTA 和植物血凝素 12 小时后添加 Zn(2+)，则 EDTA 对掺入的抑制不会完全逆转。对延迟添加 EDTA 和 Zn(2+) 影响的时间过程的研究表明，平均而言，细胞在添加植物血凝素后 20 至 30 小时内需要 Zn(2+)，以便在 37 小时时实现胸苷完全掺入。 5.植物血凝素引起的蛋白质合成速率的增加直到约8小时才被EDTA抑制。此后的进一步增加被 EDTA 完全抑制，但这种抑制可被 Zn(2+) 完全逆转。 40 小时时 EDTA 抑制培养物中的蛋白质合成速率与 10 小时时相同。 6. EDTA 对淋巴细胞刺激的影响与 Kay 等人报道的效果非常相似。 (1969) 使用低剂量的放线菌素 D。
1. Incorporation of [(3)H]thymidine into DNA was inhibited by EDTA and diethylenetriamine-NNN'N''N''-penta-acetate but not by nitrilotriacetate even when Ca(2+) was present at more than twice the concentration of the chelators. 2. The inhibition caused by EDTA was most effectively reversed by Zn(2+) but also to a lesser extent by Cd(2+). Very little if any activation of the EDTA-inhibited system was obtained with Co(2+), Cu(2+), Fe(3+), Mn(2+) or Ni(2+) added alone. 3. Fe(3+) added to the Zn(2+)-activated lymphocytes in the presence of EDTA markedly increased thymidine incorporation. Addition of Cd(2+) prevented the inhibition of incorporation which occurred at high Zn(2+) concentrations. 4. If EDTA was added more than 15h after phytohaemagglutinin, the inhibition of incorporation was less than that obtained by its addition at zero time. If Zn(2+) was added later than 12h after EDTA and phytohaemagglutinin, the inhibition of incorporation by EDTA was not fully reversed. A study of the time-course of the effects of delayed additions of EDTA and Zn(2+) suggested that, on average, the cells required Zn(2+) between 20 and 30h after phytohaemagglutinin addition to allow the full rate of thymidine incorporation at 37h. 5. The increase in the rate of protein synthesis caused by phytohaemagglutinin was not inhibited by EDTA until about 8h. The further increase after this was totally inhibited by EDTA but this inhibition was fully reversible with Zn(2+). The rate of protein synthesis in EDTA-inhibited cultures at 40h was the same as that at 10h. 6. There was a close similarity between the effects of EDTA on lymphocyte stimulation and those reported by Kay et al. (1969) with low doses of actinomycin D.