KINETICS AND LOCATION OF POLIOVIRUS MACROMOLECULAR-SYNTHESIS IN CORRELATION TO VIRUS-INDUCED CYTOPATHOLOGY
KINETICS AND LOCATION OF POLIOVIRUS MACROMOLECULAR-SYNTHESIS IN CORRELATION TO VIRUS-INDUCED CYTOPATHOLOGY
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DOI：
10.1016/0042-6822(80)90530-9
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发表时间：
1980-01-01
期刊：
影响因子：
3.7
通讯作者：
BOSSART, W
中科院分区：
文献类型：
--
作者：
BIENZ, K;EGGER, D;BOSSART, W
在检测到病毒诱导的宿主细胞改变之前，病毒蛋白合成达到最大。对于 HEp-2 [人表皮样癌]细胞中的 I 型脊髓灰质炎病毒 (Mahoney)，这种情况发生在注射后大约 2.5 小时。 [感染后]。 30-60 分钟后，病毒 RNA 最大合成发生，该合成仅发生在新形成的液泡的离散区域中。随后，在仍在合成病毒 RNA 的同时，各个空泡中心合并形成越来越多的空泡，从而形成小核糖核酸病毒诱导的 c.p.e. 的典型特征。 [细胞病变效应]出现。病毒 RNA 合成（而非蛋白质合成）在结构上与细胞病理学的发生和增强密切相关。
Viral protein synthesis is maximum before virus-induced host cell alterations can be detected. With poliovirus type I (Mahoney) in HEp-2 [human epidermoid carcinoma] cells this occurs at approximately 2.5 h p.i. [post-infection]. At 30-60 min later maximal viral RNA synthesis occurs which takes place exclusively in discrete areas of newly formed vacuoles. Subsequently, while still synthesizing viral RNA, the individual vacuolated centers coalesce to form an increasing mass of vacuoles so that the typical aspect of picornavirus-induced c.p.e. [cytopathic effect] emerges. Viral RNA synthesis, but not protein synthesis, is structurally tightly connected with the onset and increase of cytopathology.