Murine model of prosthesis failure for the long-term study of aseptic loosening
Murine model of prosthesis failure for the long-term study of aseptic loosening
复制标题
DOI：
10.1002/jor.20342
复制
发表时间：
2007-05-01
影响因子：
2.8
通讯作者：
Wooley, Paul H.
中科院分区：
文献类型：
--
作者：
Yang, Shang-You;Yu, Haiying;Wooley, Paul H.
我们研究了一种新的磨损碎屑诱导的假体不稳定和骨质溶解的小鼠模型，及其在治疗评价中的应用。将不锈钢或钛合金针植入胫骨近端，与关节软骨形成连续表面。在一些小鼠中，在手术期间将钛颗粒注射到胫骨管中，然后每月进行关节内注射。MicroCT扫描显示，无颗粒挑战的种植体稳定，6个月内无骨密度变化。组织学分析显示术后6周植入物周围有新骨形成。在暴露于钛颗粒的样本中，假体周围软组织伴炎症细胞是植入物和周围骨之间界面处的普遍发现，IL-1 β、TNF α和CD 68的表达在这些关节中很常见。拔出测试表明，需要平均5 N的载荷才能将稳定的植入物从周围骨中拔出。然而，颗粒刺激显著降低了拔出力至小于0.4 N。通过在假体关节中注射AAV-LacZ后滑膜和假体周围组织的X-gal染色证实了在该模型上体内基因转移的可行性。这种负重膝关节假体的小鼠模型为研究关节置换术失败提供了一种经济、可重复且易于获得的方法。除了组织学和生物化学检查之外，还能够评价人工关节的生物力学特性，这导致了一种有用的模型，用于研究人工关节组件在对碎片相关骨质溶解的反应期间的许多特性。(c)2007年，任研究院院长。
We examined a novel mouse model of wear debris-induced prosthesis instability and osteolysis, and its application for the evaluation of therapy. A stainless steel or titanium-alloy pin was implanted into the proximal tibia to form a contiguous surface with the articular cartilage. In some mice, titanium particles were injected into the tibial canal during the surgery, followed by monthly intraarticular injection. MicroCT scans revealed that the implants without particle challenge were stable without bone mineral density changes for 6 months. Histological analysis showed new bone formation around the implant at 6 weeks postsurgery. Periprosthetic soft tissue with inflammatory cells was a ubiquitous finding at the interface between the implant and surrounding bone in samples exposed to titanium particles, and expression of IL-1 beta, TNF alpha, and CD68 was common in these joints. Pullout tests indicated that an average 5N load was required to pull out stable implants from surrounding bone. However, particle stimulation dramatically reduced the pullout force to less than 0.4 N. The feasibility of in vivo gene transfer on this model was confirmed by X-gal staining of synovial membrane and periprosthetic tissue after injection of AAV-LacZ in the prosthetic joint. This murine model of weight-bearing knee prosthesis provides an economical, reproducible, and easily obtained means to study joint arthroplasty failure. The ability to evaluate the biomechanical properties of the prosthetic joint, in addition to histological and biochemical examination, results in a useful model to investigate many of the properties of prosthetic joint components during the response to debris-associated osteolysis. (c) 2007 Ortbopaedic Research Society.