Glial aromatization decreases neural injury in the zebra finch (Taeniopygia guttata): influence on apoptosis.
Glial aromatization decreases neural injury in the zebra finch (Taeniopygia guttata): influence on apoptosis.
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胶质芳香化减少斑胸草雀（Taeniopygia guttata）的神经损伤：对细胞凋亡的影响。
DOI：
10.1111/j.1365-2826.2004.01217.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Saldanha,CJ
中科院分区：
文献类型：
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作者：
Wynne,RD;Saldanha,CJ
新的证据表明雌激素在脊椎动物大脑受损后具有神经保护作用。芳香酶（雌激素合酶）在几种脊椎动物神经损伤区域周围的神经胶质细胞中快速转录和翻译。然而，损伤周围局部上调的神经胶质芳香酶所提供的潜在神经保护作用仍有待测试。为此，个别鸟类通过含有载体或芳香酶抑制剂法屈唑的针进入对侧半球遭受穿透性机械损伤。七十二小时后，评估神经损伤的大小（通过坏死组织的范围来评估）和损伤周围凋亡细胞的数量。注射法屈唑的半球损伤大小明显大于注射媒介物造成的损伤。此外，相对于载体，在法屈唑相关病变周围发现了更多数量的凋亡核。最后，接近损伤部位的神经元芳香酶的组成型表达在半球之间没有差异。我们得出的结论是，损伤部位周围神经胶质芳香酶的局部抑制在鸣禽大脑中发挥神经保护作用，并且这种保护涉及细胞凋亡途径。神经胶质芳香酶的局部上调可能在限制继发性损伤和/或加速脊椎动物脑损伤后的恢复过程中发挥关键作用。
Emerging evidence suggests a neuroprotective role for oestrogens following damage to the vertebrate brain. Aromatase (oestrogen synthase) is rapidly transcribed and translated in glial cells around areas of neural damage in several vertebrates. However, the potential neuroprotection afforded by locally up‐regulated glial aromatase immediately surrounding the injury remains to be tested. Towards this end, individual birds sustained penetrating mechanical injuries via a needle that contained either vehicle or the aromatase inhibitor fadrozole into contralateral hemispheres. Seventy‐two hours later, the size of neural injury (as assessed by the extent of necrotic tissue) and the number of apoptotic cells around the injuries were evaluated. The size of injury in the hemisphere injected with fadrozole was significantly larger than the injury caused by vehicle injection. Furthermore, a greater number of apoptotic nuclei were found around the fadrozole‐associated lesion relative to vehicle. Finally, constitutively expressed, neuronal aromatase close to the injury site did not differ between hemispheres. We conclude that local inhibition of glial aromatase immediately around the site of injury plays a neuroprotective role in the songbird brain and this protection involves apoptotic pathways. Local up‐regulation of glial aromatase may play a pivotal role in the limitation of secondary damage and/or the acceleration of restorative processes following injury to the vertebrate brain.