Impact of dietary fatty acids on metabolic activity and host intestinal microbiota composition in C57BL/6J mice
Impact of dietary fatty acids on metabolic activity and host intestinal microbiota composition in C57BL/6J mice
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DOI：
10.1017/s0007114514000117
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发表时间：
2014-06-14
影响因子：
3.6
通讯作者：
Stanton, Catherine
中科院分区：
文献类型：
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作者：
Patterson, Elaine;O' Doherty, Robert M.;Stanton, Catherine
不同的膳食脂肪和能量亚型对宿主的代谢健康和肠道微生物群都有影响。本研究评估了膳食脂肪质量的影响，重点关注不同饱和度的膳食脂肪酸组成、对宿主代谢健康状况和肠道微生物群组成的影响。 C57BL/6J 小鼠（每组 9-10 只小鼠）被喂食含有 (1) 棕榈油、(2) 橄榄油、(3) 红花油或 (4) 亚麻籽/鱼油的高脂肪 (HF) 饮食，持续 16 周，并与喂食补充有高玉米淀粉或高蔗糖的低脂 (LF) 饮食的小鼠进行比较。通过 GLC 评估组织脂肪酸组成，并使用高通量 16S rRNA 测序研究饮食对宿主肠道微生物群的影响。成分测序分析显示，与膳食中补充橄榄油相比，膳食中补充棕榈油导致拟杆菌门水平显着降低（P
Different dietary fat and energy subtypes have an impact on both the metabolic health and the intestinal microbiota population of the host. The present study assessed the impact of dietary fat quality, with a focus on dietary fatty acid compositions of varying saturation, on the metabolic health status and the intestinal microbiota composition of the host. C57BL/6J mice (n 9-10 mice per group) were fed high-fat (HF) diets containing either (1) palm oil, (2) olive oil, (3) safflower oil or (4) flaxseed/fish oil for 16 weeks and compared with mice fed low-fat (LF) diets supplemented with either high maize starch or high sucrose. Tissue fatty acid compositions were assessed by GLC, and the impact of the diet on host intestinal microbiota populations was investigated using high-throughput 16S rRNA sequencing. Compositional sequencing analysis revealed that dietary palm oil supplementation resulted in significantly lower populations of Bacteroidetes at the phylum level compared with dietary olive oil supplementation (P