Quantitative analysis of heterogeneous spatial distribution of Arabidopsis leaf trichomes using micro X-ray computed tomography
Quantitative analysis of heterogeneous spatial distribution of Arabidopsis leaf trichomes using micro X-ray computed tomography
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DOI：
10.1111/j.1365-313x.2008.03609.x
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发表时间：
2008-11-01
期刊：
影响因子：
7.2
通讯作者：
Toyoda, Tetsuro
中科院分区：
文献类型：
--
作者：
Kaminuma, Eli;Yoshizumi, Takeshi;Toyoda, Tetsuro
可以基于从微X射线计算机断层扫描（mu CT）图像重建的3D解剖结构来定义定量形态学性状。在这项研究中，在拟南芥叶片近轴面上的毛（毛）的异质空间分布进行了评估的三维数量性状，包括毛的数量，毛之间的平均最近邻距离，和大毛的比例。这些数据反映了在径向方向上的空间异质性，在相对于非边缘的叶片边缘上观察到更多数量的毛状体，这是由CAPRICE（CPC）和GLABRA3（GL3）基因引起的分布效应，其先前已被证明影响毛状体密度。我们进一步确定，在野生型植物和GL3突变体中，叶片中脉上的大毛状体的比例从近端到远端叶尖增加。我们的研究结果表明，GL3基因影响毛状体的分布，而不是毛状体的生长，导致毛状体的起始在近端的基础，而不是远端。另一方面，CPC确实影响毛状体的生长和发育进程。因此，基于μ CT的定量表型分析能够精确地描述表型，以阐明形态突变体中的基因控制。
Quantitative morphological traits may be defined based on the 3D anatomy reconstructed from micro X-ray computed tomography (mu CT) images. In this study, the heterogeneous spatial distribution of trichomes (hairs) on the adaxial leaf blade surface in Arabidopsis was evaluated in terms of 3D quantitative traits, including trichome number, average nearest-neighbour distance between trichomes, and proportion of large trichomes. The data reflect spatial heterogeneity in the radial direction, in that a greater number of trichomes were observed on the leaf blade margins relative to the non-margins, a distribution effect caused by the CAPRICE (CPC) and GLABRA3 (GL3) genes, which have previously been shown to affect trichome density. We further determined that the proportion of large trichomes on the blade mid-rib increases from the proximal end to the distal leaf tip in both wild-type plants and GL3 mutants. Our results indicate that the GL3 gene affects trichome distribution, rather than trichome growth, causing trichome initiation at the proximal base rather than the distal tip. On the other hand, CPC does affect trichome growth and developmental progression. Hence, quantitative phenotyping based on mu CT enables precise phenotypic description for elucidation of gene control in morphological mutants.