The Ultrasound Elastic Imaging Monitoring of the Injectable Chitosan/Nano-Hydroxyapatite/Collagen Bone Scaffolds and Comparisons with Micro-Computed Tomography and Histological Changes
The Ultrasound Elastic Imaging Monitoring of the Injectable Chitosan/Nano-Hydroxyapatite/Collagen Bone Scaffolds and Comparisons with Micro-Computed Tomography and Histological Changes
复制标题
可注射壳聚糖/纳米羟基磷灰石/胶原骨支架的超声弹性成像监测及与显微计算机断层扫描和组织学变化的比较
DOI：
10.1166/jbt.2016.1472
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发表时间：
2016-07
影响因子：
0.1
通讯作者：
Yu Bo
中科院分区：
文献类型：
--
作者：
Chen Yan;Liu Rui;Gong Yong;Wu XiXi;Huang Zhi;Dong Chao;Li Chentian;Yu Bo
新的评价方法来评估可注射骨生物材料原位成骨性能是非常必要的。在这项研究中，采用超声弹性成像（UEI）来测量可注射骨生物材料在体内的能力。皮下注射后，通过UEI扫描评价新型温敏可注射壳聚糖/纳米羟基磷灰石/胶原（CS/nHAC）支架和纯壳聚糖（CS）支架在0，1，2，4，6和8周。在第4周和第8周收集标本进行显微CT和组织学分析。通过UEI可以很好地测量可注射支架的刚度变化。此外，UEI所揭示的可注射支架的刚度、体积和血流量的动态变化可以通过显微CT和组织学分析的结果来解释。UEI能很好地监测可注射骨支架在体内的成骨和血管化变化的力学行为，为可注射骨生物材料的进一步研究提供了一种新的评价方法。
Novel evaluation methods to assess the osteogenetic properties of injectable bone biomaterials in situ are quite imperative. In this study, ultrasound elastic imaging (UEI) was employed to measure the capacity of injectable bone biomaterials in vivo. After subcutaneous injection, a novel thermosensitive injectable chitosan/nano-hydroxyapatite/collagen (CS/nHAC) scaffold and the pure chitosan (CS) scaffold were evaluated by a UEI scan at week 0, 1, 2, 4, 6 and 8. The specimens were harvested at week 4 and 8 for micro-CT and histological analyses. The stiffness variation of the injectable scaffolds was well measured by UEI. Moreover, the dynamic changes in the stiffness, volume and blood flow of the injectable scaffolds revealed by UEI could be expectedly and correspondingly explained by the results from the micro-CT and histology analyses. UEI could well monitor the mechanical behavior of the ossificational and vascularizational changes of the injectable scaffolds in vivo, thereby providing a novel assessment method for the further research of the injectable bone biomaterials.