GC-MS/MS detects potential pregabalin abuse in susceptible subjects' hair
GC-MS/MS detects potential pregabalin abuse in susceptible subjects' hair
复制标题
DOI：
10.1002/dta.2347
复制
发表时间：
2018-06-01
影响因子：
2.9
通讯作者：
Gambelunghe, Cristiana
中科院分区：
文献类型：
--
作者：
Ianni, Federica;Aroni, Kyriaki;Gambelunghe, Cristiana
普瑞巴林是一种GABA类似物，与电压依赖性钙通道的α 2 δ亚基结合。它被认为是有效的病理，如癫痫，神经性疼痛，焦虑症。由于普瑞巴林处方在全球范围内增加，其滥用的报告不断积累，主要是在阿片类药物滥用障碍患者中。本研究通过毛发分析调查了潜在的普瑞巴林滥用，这是一个提供有价值的回顾性信息的矩阵。在280名易受感染的患者中，有一半是偶尔吸毒者，正在监测驾驶执照的更新情况。其他140名患者有阿片类药物依赖史，并进行监测，以评估美沙酮治疗的依从性。针对头发样品中普瑞巴林的测定，采用甲醇提取，成功地衍生化得到氯甲酸乙酯衍生物，最后用气相色谱-串联质谱法进行分析。评价了定量方法的选择性、线性、检测限、定量限、回收率、日内和日间精密度和准确度。普瑞巴林的检测限和定量限分别为30 pg/mg和50 pg/mg。我们发现，10.7%的美沙酮患者和4.29%的偶尔吸毒者的头发样本阳性普瑞巴林没有医疗处方。头发中的平均普瑞巴林浓度高于有医学指征的消费者（1.45 ng/mg vs 0.74 ng/mg）。这些结果表明，普瑞巴林具有显着的滥用潜力，特别是在个人参加阿片类药物依赖服务，普瑞巴林滥用是一个严重的新出现的问题，应仔细监测。
Pregabalin, a GABA analogue, binds to the alpha 2 delta subunit of voltage-dependent calcium channels. It is recognised as efficacious in pathologies such as epilepsy, neuropathic pain, and anxiety disorders. Since pregabalin prescriptions have increased worldwide, reports of its abuse have been accumulating, mainly in patients with opioid abuse disorders. The present study investigated potential pregabalin abuse by means of hair analysis, a matrix that provides valuable retrospective information. Half of the pool of 280 susceptible patients had been occasional drug users and were being monitored for driving licence renewals. The other 140 patients had a history of opiate dependency and were monitored to assess compliance with methadone therapy. In view of determining pregabalin in hair samples, it was extracted in methanol, successfully derivatised to give the ethyl chloroformate derivative, and finally pregabalin was analysed by gas chromatography-tandem mass spectrometry. Selectivity, linearity, limit of detection, limit of quantification, recovery, intra- and inter-day precision, and accuracy of the quantification procedure were appraised. Pregabalin limits of detection and quantification were 30pg/mg and 50pg/mg, respectively. We found 10.7% of hair samples from methadone patients and 4.29% from occasional drug users were positive to pregabalin without medical prescription. The mean pregabalin concentration in hair was higher than in consumers with medical indications (1.45ng/mg vs 0.74ng/mg). These results suggest that pregabalin possesses a significant abuse potential particularly among individuals attending opiate dependence services and that pregabalin abuse is a serious emerging issue, which should be carefully monitored.