Two cytochromes P450 catalyze S-heterocyclizations in cabbage phytoalexin biosynthesis.
Two cytochromes P450 catalyze S-heterocyclizations in cabbage phytoalexin biosynthesis.
复制标题
两种细胞色素P450催化了卷心菜植物甲状腺素生物合成中的S-杂细胞。
DOI：
10.1038/nchembio.1914
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发表时间：
2015-11
影响因子：
14.8
通讯作者：
Sattely ES
中科院分区：
文献类型：
--
作者：
Klein AP;Sattely ES
植物补体蛋白在可食用十字花科植物中含量丰富，具有重要的生物活性，但其生物合成的专一性基因尚不清楚。在这里，我们报道了来自白菜的两种新的细胞色素P450，它们催化了前所未有的环油菜素和螺油菜素生物合成中的S杂环化反应。我们的结果首次揭示了一对重要的饮食代谢物的生物合成的遗传学和生化洞察力，并对最近测序的20种十字花科植物的途径发现具有启示意义。
Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results reveal the first genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites, and have implications for pathway discovery across >20 recently sequenced crucifers.