Further extension of mammalian GATA‐6
Further extension of mammalian GATA‐6
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DOI：
10.1111/j.1440-169x.2005.00837.x
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发表时间：
2005-12
期刊：
影响因子：
4.6
通讯作者：
M. Maeda;K. Ohashi;Ayako Ohashi-Kobayashi
中科院分区：
文献类型：
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作者：
M. Maeda;K. Ohashi;Ayako Ohashi-Kobayashi
哺乳动物加塔-6具有保守的串联锌指（CVNC-X17-CNAC）-X29-（CXNC-X17-CNAC），对心脏、胃肠道和其他组织的发育和特异性基因调控至关重要。加塔-6识别（A/T/C）GAT（A/T）（A）序列，并通过其锌指区与其他转录调控因子相互作用。加塔-6的mRNA使用两个符合读框的Met密码子作为翻译起始密码子，并通过漏核糖体扫描产生L-和S-型加塔-6。加塔-6在异源表达系统中通过蛋白酶体进行cAMP依赖性蛋白水解。加塔-6的这些基于蛋白质的特征将有助于靶基因的鉴定，以及加塔-6的体内结合位点的确定和对加塔-6介导的基因调控的复杂网络的理解。
Mammalian GATA‐6, which has conserved tandem zinc fingers (CVNC‐X17‐CNAC)‐X29‐(CXNC‐X17‐CNAC), is essential for the development and specific gene regulation of the heart, gastrointestinal tract and other tissues. GATA‐6 recognizes the (A/T/C)GAT(A/T)(A) sequence, and interacts with other transcriptional regulators through its zinc‐finger region. The mRNA of GATA‐6 uses two Met codons in frame as translational initiation codons, and produces L‐ and S‐type GATA‐6 through leaky ribosome scanning. GATA‐6 is subjected to cAMP‐dependent proteolysis by a proteasome in a heterologous expression system. These protein‐based characteristics of GATA‐6 will be helpful for the identification of target genes, together with determination of the in vivo binding sites for GATA‐6 and understanding of the complex network of gene regulation mediated by GATA‐6.