IL‐1α, IL‐1β, IL‐6, and TNF‐α Steady‐State mRNA Levels Analyzed by Reverse Transcription‐Competitive PCR in Bone Marrow of Gonadectomized Mice
IL‐1α, IL‐1β, IL‐6, and TNF‐α Steady‐State mRNA Levels Analyzed by Reverse Transcription‐Competitive PCR in Bone Marrow of Gonadectomized Mice
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通过逆转录竞争性 PCR 分析性腺切除小鼠骨髓中 IL-1α、IL-1β、IL-6 和 TNF-α 稳态 mRNA 水平
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
C. Löwik
中科院分区：
文献类型：
--
作者：
R. van Bezooijen;H. Farih‐Sips;S. Papapoulos;C. Löwik
女性和男性的性腺功能丧失都与骨质流失率增加有关，其机制尚不清楚。有充分的证据表明骨微环境中产生并刺激破骨细胞的活性和/或形成的细胞因子参与其中。在本研究中，我们检查了性腺切除术是否通过增加小鼠骨髓中的转录来增加细胞因子的产生。为此，通过逆转录竞争性聚合酶链反应测定了卵巢切除或睾丸切除后不同时间点小鼠中央骨髓室中多种细胞因子的体内稳态 mRNA 水平。 mRNA 表达的可检测差异的极限约为 2 倍。用脂多糖治疗小鼠后，细胞因子白细胞介素-1α (IL-1α)、白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6) 和肿瘤坏死因子-α (TNF-α) 的骨髓 mRNA 水平升高达 30 倍。性腺切除术后 4、7 和 14 天雌性和雄性小鼠骨髓中这些细胞因子的 mRNA 表达没有差异。因此，性别类固醇缺乏不会增加小鼠中央骨髓室细胞中 IL-1α、IL-1β、IL-6 和 TNF-α 的稳态 mRNA 水平。如果发生变化，变化应该小于 2 倍，或者发生在小细胞群中。这些结果并不排除这些细胞因子在性腺切除术后诱导骨质流失中的重要作用。例如，靠近骨表面的骨髓细胞或骨细胞可能导致细胞因子合成增加。或者，性别类固醇的丧失可能会改变细胞因子合成和活性的转录后事件，或者可能改变靶细胞的反应性。
Loss of gonadal function in both females and males is associated with increased rates of bone loss by a yet unidentified mechanism. There is ample evidence that cytokines that are produced in the bone microenvironment and stimulate the activity and/or formation of osteoclasts are involved. In the present study, we examined whether gonadectomy increases cytokine production via increased transcription in the bone marrow of mice. For this, the in vivo steady‐state mRNA levels of multiple cytokines were determined in the central bone marrow compartment of mice at different time points following ovariectomy or orchidectomy by reverse transcription‐competitive polymerase chain reaction. The limit of detectable differences in mRNA expression was approximately 2‐fold. Bone marrow mRNA levels of the cytokines interleukin‐1α (IL‐1α), interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α) were elevated up to 30‐fold after treatment of mice with lipopolysaccharide. Following gonadectomy, there were no differences in the mRNA expression of these cytokines in bone marrow of female and male mice 4, 7, and 14 days after surgery. Gender steroid deficiency does not, therefore, increase steady‐state mRNA levels of IL‐1α, IL‐1β, IL‐6, and TNF‐α in cells of the central bone marrow compartment in mice. If changes have occurred these should have been less than 2‐fold or in a small cell population. These results do not preclude an important role of these cytokines in the induction of bone loss after gonadectomy. For example, bone marrow cells situated close to the bone surface or bone cells may be responsible for increased cytokine synthesis. Alternatively, the loss of gender steroids may alter post‐transcriptional events in cytokine synthesis and activity or may modify the responsiveness of target cells.
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影响因子：
4.8
作者：
R. Kimble;A. Matayoshi;J. Vannice;V. Kung;C. Williams;R. Pacifici
通讯作者：
R. Kimble;A. Matayoshi;J. Vannice;V. Kung;C. Williams;R. Pacifici
雌激素停药后，小鼠骨髓和骨细胞产生的白细胞介素 6 增加。
影响因子：
4.8
作者：
Passeri,G;Girasole,G;Jilka,RL;Manolagas,SC
通讯作者：
Manolagas,SC
影响因子：
15.9
作者：
KITAZAWA, R;KIMBLE, RB;PACIFICI, R
通讯作者：
PACIFICI, R
DOI：
10.1073/pnas.88.12.5134
发表时间：
1991-06-01
影响因子：
11.1
作者：
PACIFICI, R;BROWN, C;AVIOLI, LV
通讯作者：
AVIOLI, LV
影响因子：
15.9
作者：
KIMBLE, RB;VANNICE, JL;PACIFICI, R
通讯作者：
PACIFICI, R