A six-microRNA panel in plasma was identified as a potential biomarker for lung adenocarcinoma diagnosis.
A six-microRNA panel in plasma was identified as a potential biomarker for lung adenocarcinoma diagnosis.
复制标题
血浆中的六微RNA组被确定为肺腺癌诊断的潜在生物标志物
DOI：
10.18632/oncotarget.14311
复制
发表时间：
2017-01-24
期刊：
影响因子：
--
通讯作者：
Shu Y
中科院分区：
文献类型：
--
作者：
Zhou X;Wen W;Shan X;Zhu W;Xu J;Guo R;Cheng W;Wang F;Qi LW;Chen Y;Huang Z;Wang T;Zhu D;Liu P;Shu Y
肺腺癌（LA）患者血浆中特异性表达的microRNA（miRNAs）可作为LA检测的生物标志物。在筛选阶段，使用RT-qon panel进行miRNA表达谱分析，然后使用定量逆转录聚合酶链反应（qRT-PCR）进行验证（30例LA vs. 10例健康对照（HC））。通过使用基于qRT-PCR的绝对定量方法，通过训练（42个LA vs. 32个HC）和测试阶段（66个LA vs. 62个HC）确认了识别的miRNA。共鉴定出六种上调的血浆miRNA（miR-19 b-3 p、miR-21- 5 p、miR-221- 3 p、miR-409- 3 p、miR-425- 5 p和miR-584- 5 p）。六个miRNA组可以区分LA患者与HC，在训练、测试和外部验证阶段的受试者工作特征曲线下面积分别为0.72、0.74和0.84（33个LA与30个HC）。除miR-584- 5 p外，其他所有miRNA在LA组织中均显著上调。miR-19- 3 p、miR-21- 5 p、miR-409- 3 p和miR-425- 5 p在动脉血浆中显示具有边界显著性的高表达。此外，在从LA外周血浆样品提取的外来体中，miR-19- 3 p、miR-21- 5 p和miR-221- 3 p显著上调。总之，我们在外周血中鉴定了一组六种miRNA，至少在一定程度上可能有助于亚洲人群LA的检测。
Differently expressed microRNAs (miRNAs) in the plasma of lung adenocarcinoma (LA) patients might serve as biomarkers for LA detection. MiRNA expression profiling was performed using Exiqon panels followed by the verification (30 LA VS. 10 healthy controls (HCs)) with quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the screening phase. Identified miRNAs were confirmed through training (42 LA VS. 32 HCs) and testing stages (66 LA VS. 62 HCs) by using qRT-PCR based absolute quantification methods. A total of six up-regulated plasma miRNAs (miR-19b-3p, miR-21-5p, miR-221-3p, miR-409-3p, miR-425-5p and miR-584-5p) were identified. The six-miRNA panel could discriminate LA patients from HCs with areas under the receiver operating characteristic curve of 0.72, 0.74 and 0.84 for the training, testing and the external validation stage (33 LA VS. 30 HCs), respectively. All the miRNAs identified except miR-584-5p were significantly up-regulated in LA tissues. MiR-19-3p, miR-21-5p, miR-409-3p and miR-425-5p showed high expression in arterial plasma with borderline significance. Additionally, miR-19-3p, miR-21-5p and miR-221-3p were significantly up-regulated in exosomes extracted from LA peripheral plasma samples. In conclusion, we identified a six-miRNA panel in peripheral plasma which might give assistance to the detection of LA at least for Asian population to a certain extent.