Studies on the heterogeneity of hemoglobin. XVI. Separation of variants with a GlU-Lys substitution by chromatography on CM-cellulose.
Studies on the heterogeneity of hemoglobin. XVI. Separation of variants with a GlU-Lys substitution by chromatography on CM-cellulose.
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血红蛋白异质性的研究。
DOI：
10.1016/s0021-9673(00)85750-7
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
R. N. Wrightstone
中科院分区：
文献类型：
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作者：
T. Huisman;R. N. Wrightstone
通过CM-纤维素色谱法分析了6种β链变体、2种α链变体和1种δ链变体（各在不同位置用谷氨酰残基取代赖氨酰残基）以及Hb-C-哈莱姆、Hb-A2和Hb-Miyada。这些变异体可分为以下两组：I组：Hb-C、C-Chad raj、A2-墨尔本、Chad ogi、O-Arab、O-Indonesia和C-Harlem; II组：Hb-E-萨斯卡通、Hb-Chad、Hb-E、Hb-A2和Hb-Miyada。组I的变体在与Hb-A相差0.3-0.43个pH单位的洗脱pH值下洗脱，而组II的变体的这种差异仅为0.2个pH单位。这些观察结果已经根据我们目前对血红蛋白分子结构的了解进行了讨论。许多变体的洗脱pH值与Hb-A2的洗脱pH值之间的差异使得可以使用该技术来定量含有血红蛋白的红细胞溶血产物中的Hb-A2，所述血红蛋白具有与Hb-A2相似的电泳迁移率。
Six β chain variants, two α chain variants, and one δ chain variant each with a substitution of a glutamyl residue for a lysyl residue in different positions, and Hb-C-Harlem, Hb-A2, and Hb-Miyada have been analyzed by CM-cellulose chromatography. These variants can be divided into two groups as follows: Group I C, C-Siriraj, A2-Melbourne, Agenogi, O-Arab, O-Indonesia, and C-Harlem; Group II Hb-E-Saskatoon, Hb-Chad, Hb-E, Hb-A2, and Hb-Miyada. The variants of group I are eluted at an elution pH value which differs 0.3–0.43 pH units from that of Hb-A, whereas this difference for the variants of Group II is only 0.2 pH units. These observations have been discussed in the light of our present knowledge of the structure of the hemoglobin molecule. The difference between the elution pH values of many variants and that of Hb-A2makes it possible to use this technique for the quantitation of Hb-A2in red cell hemolysates containing hemoglobins which have electrophoretic mobilities similar to that of Hb-A2.