④Moyu Goto, Narumi Toda, Kouhei Shimaji, Dang Ngoc-Anh Suong, Nicole Vo, Hiroshi Kimura, Hideki Yoshida, Yoshihiro H. Inoue and Masamitsu Yamaguchi.
④Moyu Goto, Narumi Toda, Kouhei Shimaji, Dang Ngoc-Anh Suong, Nicole Vo, Hiroshi Kimura, Hideki Yoshida, Yoshihiro H. Inoue and Masamitsu Yamaguchi.
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④后藤茂友、户田成美、岛地耕平、Dang Ngoc-Anh Suong、Nicole Vo、Hiroshi Kimura、Hideki Yoshida、Yoshihiro H. Inoue 和 Masamitsu Yamaguchi。
DOI：
10.1080/21565562.2016.1232023
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Yoshihiro H. Inoue and Masamitsu Yamaguchi.
中科院分区：
文献类型：
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作者：
Moyu Goto;Narumi Toda;Kouhei Shimaji;Dang Ngoc-Anh Suong;Nicole Vo;Hiroshi Kimura;Hideki Yoshida;Yoshihiro H. Inoue and Masamitsu Yamaguchi.
果蝇Jumonji/Jarid2(DJmj)已被鉴定为多梳抑制复合体2的一个组分。然而，有人认为dJmj既有PRC依赖的作用，也有非独立的作用。DJmj在精子发生过程中的亚细胞定位尚不清楚。因此，我们用dJmj的特异性抗体和组蛋白H3(H3K27me3)上27位赖氨酸的三甲基化进行了免疫细胞化学分析。有趣的是，dJmj仅定位于生长后期的核仁。在生长后期对不同的Polycomb group(PcG)突变系的djmj定位的研究，使包括Polycomb(Pc)在内的一些PcG基因得以鉴定，这些基因负责djmj向核仁的募集。此外，我们还发现，在这些突变株系中，有一些核仁的大小减小。在负责Pc核仁定位的睾丸特异TAF同源物突变体(TTAF)中，dJmj信号不仅在核仁上检测到，而且在生长后期的浓缩染色质上也检测到。DuolinkIn情景邻近连接实验表明，在生长后期，Pc与dJmj在核仁上相互作用。此外，H3K27me3在细胞核中的水平在这一阶段下降。综上所述，我们得出结论，tTAF负责dJmj在生长后期向核仁的募集，这似乎是由Pc介导的。核仁中的dJmj和一些PcG的区隔对于在随后的减数分裂中抑制细胞生长和增殖所需的基因的表达可能是必要的。
DrosophilaJumonji/Jarid2 (dJmj) has been identified as a component of Polycomb repressive complex 2. However, it is suggested that dJmj has both PRC-dependent and –independent roles. Subcellular localization of dJmj during spermatogenesis is unknown. We therefore performed immunocytochemical analyses with specific antibodies to dJmj and tri-methylation at lysine 27 on histone H3 (H3K27me3). Interestingly, dJmj exclusively localizes at nucleolus in the late growth stage. Examination of the dJmj localization in various Polycomb group (PcG) mutant lines at the late growth stage allowed identification of some PcG genes, including Polycomb (Pc), to be responsible for dJmj recruitment to nucleolus. In addition, we found that size of nucleolus was decreased in some of these mutant lines. In a mutant of testis-specific TAF homolog (tTAF) that is responsible for nucleolus localization of Pc, dJmj signals were detected not only at nucleolus but also on the condensed chromatin in the late growth stage. DuolinkIn situProximity ligation assay clarified that Pc interacts with dJmj at nucleolus in the late growth stage. Furthermore, the level of H3K27me3 decreased in nuclei at this stage. Taken together, we conclude that tTAF is responsible for recruitments of dJmj to nucleolus in the late growth stage that appears to be mediated by Pc. Compartmentalization of dJmj in nucleolus together with some of PcG may be necessary to de-repress the expression of genes required to cellular growth and proliferation in the following meiotic divisions.