Activity-based protein profiling in microbes and the gut microbiome.
Activity-based protein profiling in microbes and the gut microbiome.
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DOI：
10.1016/j.cbpa.2023.102351
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发表时间：
2023-07
影响因子：
7.8
通讯作者：
Lin Han;Pamela V. Chang
中科院分区：
文献类型：
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作者：
Lin Han;Pamela V. Chang
基于活性的蛋白质图谱(ABPP)是研究复杂生物系统中蛋白质功能和酶活性的一种强有力的化学方法。这一策略通常利用基于活性的探针，这些探针旨在结合特定的蛋白质、氨基酸残基或蛋白质家族，并通过基于反应性的弹头形成共价键。随后通过基于质谱学的蛋白质组平台进行分析，包括点击化学或基于亲和力的标记以丰富标记的蛋白质，从而能够鉴定蛋白质的功能和酶活性。ABPP促进了细菌生物学过程的阐明，新抗生素的发现，以及生理环境中宿主-微生物相互作用的表征。本文就ABPP在细菌和复杂微生物群落中的应用及其最新进展作一综述。
Activity-based protein profiling (ABPP) is a powerful chemical approach for probing protein function and enzymatic activity in complex biological systems. This strategy typically utilizes activity-based probes that are designed to bind a specific protein, amino acid residue, or protein family and form a covalent bond through a reactivity-based warhead. Subsequent analysis by mass spectrometry-based proteomic platforms that involve either click chemistry or affinity-based labeling to enrich for the tagged proteins enables identification of protein function and enzymatic activity. ABPP has facilitated elucidation of biological processes in bacteria, discovery of new antibiotics, and characterization of host–microbe interactions within physiological contexts. This review will focus on recent advances and applications of ABPP in bacteria and complex microbial communities.