Growth and invasion of human melanomas in human skin grafted to immunodeficient mice.
Growth and invasion of human melanomas in human skin grafted to immunodeficient mice.
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发表时间：
1993-08
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通讯作者：
I. Juhász;S. Albelda;D. Elder;G. Murphy;K. Adachi;D. Herlyn;I. Vályi-Nagy;M. Herlyn
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作者：
I. Juhász;S. Albelda;D. Elder;G. Murphy;K. Adachi;D. Herlyn;I. Vályi-Nagy;M. Herlyn
开发了人黑素瘤的原位模型，其中将恶性细胞注射到移植到裸小鼠和SCID小鼠的人皮肤中。黑色素瘤细胞增殖并以特征性方式侵入人皮肤移植物。6例黑色素瘤中有3例呈多发结节状生长，浸润移植物，真皮结构无明显改变，而其他黑色素瘤沿沿着胶原纤维浸润真皮，内皮血管突出。相比之下，接种到小鼠皮肤中的黑色素瘤细胞生长为不侵入小鼠真皮的弥漫性扩张结节。在人类皮肤移植物中，人类黑色素瘤细胞对人类血管具有血管生成作用，而小鼠血管仅在移植物的外周发现。肿瘤细胞侵入人体血管，7个细胞系中有4个转移到肺部，这表明该模型可用于确定体内正常和恶性人体细胞之间的相互作用。
An orthotopic model of human melanoma was developed in which malignant cells were injected into human skin grafted to nude and SCID mice. Melanoma cells proliferated and invaded the human skin grafts with characteristic patterns. Three of six melanomas grew as multiple nodules and infiltered the grafts without major architectural changes in the dermis, whereas the others invaded the dermis along collagen fibers with prominent endothelial vessels. By contrast, melanoma cells inoculated into mouse skin grew as diffusely expanding nodules that did not invade the murine dermis. In human skin grafts, human melanoma cells were angiogenic for human blood vessels, and murine vessels were only found at the periphery of grafts. Tumor cells invaded the human vessels, and four out of seven cell lines metastasized to lungs, suggesting that this model is useful to determine in vivo the interactions between normal and malignant human cells.